Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens.
Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens.
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DOI：
10.1016/j.psj.2023.102699
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发表时间：
2023-08
期刊：
影响因子：
4.4
通讯作者：
Yoshimura, Y.
中科院分区：
文献类型：
--
作者：
Nii, T.;Shinkoda, T.;Isobe, N.;Yoshimura, Y.
鸟类输卵管通过泄殖腔连接到胃肠道，在那里它暴露于肠内容物中的致病菌。因此，改善输卵管粘膜屏障功能对家禽安全生产具有重要意义。已知乳酸菌有助于增强肠道中的粘膜屏障功能，并且预期在鸡的输卵管粘膜中具有类似的效果。本研究旨在阐明阴道给药乳酸菌对输卵管粘膜屏障功能的影响。白色约氏乳杆菌（Lactobacillus johnsonii，LL）低浓度组和高浓度组（HL）分别接种1 × 105和1 × 108 cfu/mL，对照组（C组）分别接种1 mL约氏乳杆菌（Lactobacillus johnsonii，LL）和1 mL约氏乳杆菌（HL），连续7 d（n = 6）。收集输卵管大孔、子宫和阴道进行组织学观察和粘膜屏障功能相关基因表达分析。还进行了输卵管粘液细菌的扩增子序列分析。在实验期间收集鸡蛋并测量其重量。阴道施用L.约氏菌感染7 d后，1）阴道粘膜微生物群的α多样性增加，有益菌丰度比增加，致病菌丰度比减少，2）在阴道粘膜和阴道粘膜中的claudin（CLA）1和3基因表达增强，3）在阴道粘膜和子宫中的AvBD 10，11和12基因表达减少，和阴道粘膜。这些结果表明，经阴道给药L。Johnsonii通过改善输卵管粘膜的微生物区系和加强紧密连接的机械屏障功能而有助于保护输卵管免受感染。相反，经阴道给予乳酸菌不会增强输卵管中AvBD 10、11和12的产生。
The avian oviduct connects to the gastrointestinal tract through cloaca, where it is exposed to pathogenic bacteria from intestinal contents. Therefore, improvement of mucosal barrier function in the oviduct is important for safe poultry production. Lactic acid bacteria are known to contribute to strengthening the mucosal barrier function in the intestinal tract, and a similar effect is expected in the oviduct mucosa of chickens. This study aimed to clarify the effects of vaginal administration of lactic acid bacteria on the mucosal barrier function of the oviduct. White Leghorn laying hens (500-days old) were intravaginally administered 1 mL of Lactobacillus johnsonii suspension (1 × 105 and 1 × 108 cfu/mL: low concentration of Lactobacillus (LL) and high concentration of Lactobacillus (HL) groups, respectively) or without bacteria (control: C group) for 7 d (n = 6). The oviductal magnum, uterus, and vagina were collected for histological observations and mucosal barrier function-related gene expression analysis. Amplicon sequence analysis of oviductal mucus bacteria was also performed. Eggs were collected during the experimental period and their weight was measured. Vaginally administering L. johnsonii for 7 d caused 1) an increase in α-diversity of vaginal mucosa microbiota with an increase in the abundance ratio of beneficial bacteria and a decrease in pathogenic bacteria, 2) enhanced claudin (CLA) 1 and 3 gene expression in the magnum and vaginal mucosa, and 3) a decrease in avian β-defensin (AvBD) 10, 11, and 12 gene expression in the magnum, uterus, and vaginal mucosa. These results suggest that transvaginal administration of L. johnsonii contributes to protection against infection in the oviduct by improving the microflora of the oviductal mucosa and strengthening the mechanical barrier function of the tight junctions. In contrast, transvaginal administration of lactic acid bacteria does not enhance the production of AvBD10, 11, and 12 in the oviduct.
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影响因子：
6.3
作者：
Jiang, Jingle;Qi, Lina;Wei, Quanwei;Shi, Fangxiong
通讯作者：
Shi, Fangxiong
影响因子：
3.4
作者：
Ikeyama N;Murakami T;Toyoda A;Mori H;Iino T;Ohkuma M;Sakamoto M
通讯作者：
Sakamoto M
DOI：
10.1111/j.1439-0396.2009.00933.x
发表时间：
2010-08-01
影响因子：
2.7
作者：
Awad, W. A.;Ghareeb, K.;Boehm, J.
通讯作者：
Boehm, J.
影响因子：
3.7
作者：
Huang C
通讯作者：
Huang C
影响因子：
5.7
作者：
Su W;Chen Y;Cao P;Chen Y;Guo Y;Wang S;Dong W
通讯作者：
Dong W