Role of surface charge density in nanoparticle-templated assembly of bromovirus protein cages.
Role of surface charge density in nanoparticle-templated assembly of bromovirus protein cages.
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DOI：
10.1021/nn1005073
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发表时间：
2010-07-27
期刊：
影响因子：
17.1
通讯作者：
Dragnea B
中科院分区：
文献类型：
--
作者：
Daniel MC;Tsvetkova IB;Quinkert ZT;Murali A;De M;Rotello VM;Kao CC;Dragnea B
自组装二十面体蛋白质笼具有潜在的有用的物理和化学特性的各种纳米技术的应用，从治疗或诊断载体的层次材料的积木。对于蛋白质笼组件的特定应用功能控制，有必要更深入地了解蛋白质笼及其有效载荷之间的相互作用。蛋白笼封装的纳米颗粒，其明确的表面化学，允许系统控制封装的关键参数，如表面电荷，疏水性和大小。对这些变量的独立控制允许对不同的装配机构模型进行实验测试。先前对雀麦花叶病毒衣壳和带负电荷的金纳米颗粒进行的研究表明，自组装过程的结果取决于颗粒的直径。然而，在这些实验中，表面配体密度保持在饱和水平，而总电荷和曲率半径仍然耦合变量，使得难以解释所观察到的依赖于核心大小。目前的工作通过旨在解耦的表面电荷的核心大小的分析，证明了组装的临界表面电荷密度。
Self-assembling icosahedral protein cages have potencially useful physical and chemical characteristics for a variety of nanotechnology applications, ranging from therapeutic or diagnostic vectors to building blocks for hierarchical materials. For application-specific functional control of protein cage assemblies, a deeper understanding of the interaction between the protein cage and its payload is necessary. Protein-cage encapsulated nanoparticles, with their well-defined surface chemistry, allow for systematic control over key parameters of encapsulation such as the surface charge, hydrophobicity, and size. Independent control over these variables allows experimental testing of different assembly mechanism models. Previous studies done with Brome mosaic virus capsids and negatively-charged gold nanoparticles indicated that the result of the self-assembly process depends on the diameter of the particle. However, in these experiments, the surface-ligand density was maintained at saturation levels, while the total charge and the radius of curvature remained coupled variables, making the interpretation of the observed dependence on the core size difficult. The current work furnishes evidence of a critical surface charge density for assembly through an analysis aimed at decoupling the surface charge the core size.
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