Pulmonary fibrotic response to aspiration of multi-walled carbon nanotubes.
Pulmonary fibrotic response to aspiration of multi-walled carbon nanotubes.
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DOI：
10.1186/1743-8977-8-21
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发表时间：
2011-07-22
影响因子：
10
通讯作者：
Porter DW
中科院分区：
文献类型：
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作者：
Mercer RR;Hubbs AF;Scabilloni JF;Wang L;Battelli LA;Friend S;Castranova V;Porter DW
多壁碳纳米管（MWCNTs）是一种新型纳米材料，具有广泛的商业应用。为了验证MWCNTs引起持续性肺部病理的假设，将C57BL/6J小鼠通过咽部滴入暴露于10、20、40或80 μg的MWCNTs（平均尺寸为3.9 μm × 49 nm）或载体。在暴露后1、7、28和56天保存肺，以确定MWCNT肺负荷影响的潜在区域和靶细胞。采用天狼星红染色形态学测量法评估结缔组织反应。暴露后56天，肺泡巨噬细胞、肺泡组织和肉芽肿病变分别占MWCNT肺负荷的68.7±3.9、7.5±1.9和22.0±5.1%（平均±SE， N = 8）。胸膜下组织占MWCNT肺负荷的1.6%。80 μg暴露组肺泡间质结缔组织厚度随时间逐渐增加（暴露后1、7、28和56天，平均±SE， N = 8）（0.12±0.01、0.12±0.01、0.16±0.01和0.19±0.01 μm）。暴露后56天测定肺泡间隔结缔组织平均厚度为0.11±0.01,0.14±。小鼠组、10、20、40、80 μg剂量组的细胞凋亡指数分别为02、0.14±0.01、0.16±0.01、0.19±0.01 μm（平均±SE， N = 8）。肺负荷主要分布在肺泡巨噬细胞内，约8%的巨噬细胞转移到肺泡隔，而胸膜下组织的负荷较小，但可能很重要。尽管肺负荷传递到肺泡组织的比例相对较低，但与对照相比，40 μg暴露组肺泡间隔结缔组织的平均厚度增加了45%，80 μg暴露组增加了73%。结果表明，MWCNTs具有在肺肺泡组织中产生进行性纤维化反应的潜力。然而，每μg剂量的间质MWCNTs对结缔组织的增加明显低于先前发现的单壁碳纳米管（SWCNTs）。
Multi-walled carbon nanotubes (MWCNTs) are new manufactured nanomaterials with a wide spectrum of commercial applications. To address the hypothesis that MWCNTs cause persistent pulmonary pathology, C57BL/6J mice were exposed by pharyngeal aspiration to 10, 20, 40 or 80 μg of MWCNTs (mean dimensions of 3.9 μm × 49 nm) or vehicle. Lungs were preserved at 1, 7, 28 and 56 days post- exposure to determine the potential regions and target cells for impact by MWCNT lung burden. Morphometric measurement of Sirius Red staining was used to assess the connective tissue response. At 56 days post-exposure, 68.7 ± 3.9, 7.5 ± 1.9 and 22.0 ± 5.1 percent (mean ± SE, N = 8) of the MWCNT lung burden were in alveolar macrophages, alveolar tissue and granulomatous lesions, respectively. The subpleural tissues contained 1.6% of the MWCNT lung burden. No MWCNTs were found in the airways at 7, 28 or 56 days after aspiration The connective tissue in the alveolar interstitium demonstrated a progressive increase in thickness over time in the 80 μg exposure group (0.12 ± 0.01, 0.12 ± 0.01, 0.16 ± 0.01 and 0.19 ± 0.01 μm for 1, 7, 28 and 56 days post-exposure (mean ± SE, N = 8)). Dose-response determined at 56 days post-exposure for the average thickness of connective tissue in alveolar septa was 0.11 ± 0.01, 0.14 ± .02, 0.14 ± 0.01, 0.16 ± 0.01 and 0.19 ± 0.01 μm (mean ± SE, N = 8) for vehicle, 10, 20, 40 and 80 μg dose groups, respectively. The distribution of lung burden was predominately within alveolar macrophages with approximately 8% delivery to the alveolar septa, and a smaller but potentially significant burden to the subpleural tissues. Despite the relatively low fraction of the lung burden being delivered to the alveolar tissue, the average thickness of connective tissue in the alveolar septa was increased over vehicle control by 45% in the 40 μg and 73% in the 80 μg exposure groups. The results demonstrate that MWCNTs have the potential to produce a progressive, fibrotic response in the alveolar tissues of the lungs. However, the increases in connective tissue per μg dose of MWCNTs to the interstitium are significantly less than those previously found for single-walled carbon nanotubes (SWCNTs).
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影响因子：
5
作者：
Sager, Tina M.;Porter, Dale W.;Castranova, Vincent
通讯作者：
Castranova, Vincent
亚慢性暴露于多壁碳纳米管对小鼠的影响
DOI：
10.1080/15287390903523378
发表时间：
2010-01-01
影响因子：
2.6
作者：
Liang, Geyu;Yin, Lihong;Pu, Yuepu
通讯作者：
Pu, Yuepu
影响因子：
3.8
作者：
Ma-Hock, Lan;Treumann, Silke;Landsiedel, Robert
通讯作者：
Landsiedel, Robert
影响因子：
3.8
作者：
Muller, J;Huaux, F;Lison, D
通讯作者：
Lison, D
影响因子：
38.3
作者：
通讯作者：
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