Expression of five frizzleds during zebrafish craniofacial development.
Expression of five frizzleds during zebrafish craniofacial development.
复制标题
DOI：
10.1016/j.gep.2009.07.003
复制
发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Topczewski J
中科院分区：
文献类型：
--
作者：
Sisson BE;Topczewski J
WNT/平面细胞极性(PCP)信号对动物的正常发育至关重要。虽然最初在果蝇中发现了这一途径，但它对脊椎动物的正常发育也是必不可少的。斑马鱼突变体，Wnt/PCP途径缺陷，经常表现出会聚和伸展原肠运动的缺陷，以及后来的额外异常，包括头面部软骨的形态发生问题。虽然在斑马鱼中发现了多种Frizzled同系物，Wnt受体，但Fzd在形成早期幼虫头部骨骼中的作用尚不清楚。为了确定哪些斑马鱼参与了这一过程，我们分析了5个斑马鱼冷冻同系物fzd2、6、7a、7b和8a在受精后2-4天(DPF)的表达。在所分析的发育时间点，fzd2和fzd6在整个头部广泛表达，而fzd7a、7b和8a的表达受到更多的限制。进一步观察发现，fzd7b在咽弓的神经脊和中胚层核心以及新堆积的颅面软骨元素的软骨细胞中都有表达。然而，fzd7a仅在咽弓的神经脊表达，而fzd8a在咽内胚层表达。
Wnt/Planar Cell Polarity (PCP) signaling is critical for proper animal development. While initially identified in Drosophila, this pathway is also essential for the proper development of vertebrates. Zebrafish mutants, defective in the Wnt/PCP pathway, frequently display defects in convergence and extension gastrulation movements and additional later abnormalities including problems with craniofacial cartilage morphogenesis. Although multiple Frizzled (Fzd) homologues, Wnt receptors, were identified in zebrafish, it is unknown which Fzd plays a role in shaping the early larvae head skeleton. In an effort to determine which Frizzleds are involved in this process, we analyzed the expression of five zebrafish frizzled homologues fzd2, 6, 7a, 7b, and 8a from 2–4 days post fertilization (dpf). During the analyzed developmental time points fzd2 and fzd6 are broadly expressed throughout the head, while the expression of fzd7a, 7b and 8a is much more restricted. Closer examination revealed that fzd7b is expressed in the neural crest and the mesodermal core of the pharyngeal arches and in the chondrocytes of newly stacked craniofacial cartilage elements. However, fzd7a is only expressed in the neural crest of the pharyngeal arches and fzd8a is expressed in the pharyngeal endoderm.
登录
查看更多内容
影响因子：
1.5
作者：
Sumanas, S;Kim, HJ;Ekker, SC
通讯作者：
Ekker, SC
影响因子：
2.6
作者：
Ungar, AR;Calvey, CR
通讯作者：
Calvey, CR
DOI：
10.1073/pnas.0402802101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Guo, N;Hawkins, C;Nathans, J
通讯作者：
Nathans, J
影响因子：
21.3
作者：
Devenport, Danelle;Fuchs, Elaine
通讯作者：
Fuchs, Elaine
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF