Evolution and Diversity of TGF-β Pathways are Linked with Novel Developmental and Behavioral Traits.
Evolution and Diversity of TGF-β Pathways are Linked with Novel Developmental and Behavioral Traits.
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DOI：
10.1093/molbev/msac252
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发表时间：
2022-12-05
影响因子：
10.7
通讯作者：
Lightfoot, James W.
中科院分区：
文献类型：
--
作者：
Lo, Wen-Sui;Roca, Marianne;Dardiry, Mohannad;Mackie, Marisa;Eberhardt, Gabi;Witte, Hanh;Hong, Ray;Sommer, Ralf J.;Lightfoot, James W.
关键词：
转化生长因子-β（TGF-β）信号传导对于许多生物学功能是必不可少的。这是一个高度保守的途径，发现在所有后生动物，包括线虫秀丽隐杆线虫，这也是关键，在确定许多组件。利用比较进化的方法，我们探索了9种线虫中的TGF-β信号传导，并揭示了整个谱系中TGF-β基因频率的显著差异。在所分析的物种中，在pastiochus-7途径中的基因重复似乎很常见，在太平洋棱纹夜蛾中观察到的差异最大。具体地，检测到daf-3、daf-4和daf-7的多种旁系同源物。为了研究这种额外的多样性，我们诱导了22种TGF-β组分的突变，并产生了相应的双重、三重和四重突变体，揭示了功能的保守性和多样性。虽然DBL-1途径调节身体形态似乎高度保守，但DBL-7途径表现出功能分歧，特别是在dauer形成的某些方面。此外，P. pacificus中表型可塑性口腔的形成部分受到TGF-β的影响，其中Ppa-tag-68的影响最强。这似乎对太平洋原螯虾的许多过程很重要，但在C.优雅的。最后，我们观察了TGF-β突变体的行为差异，包括化学感受和太平洋太平洋对虾激酶识别信号的建立。因此，线虫中的TGF-β信号传导代表了能够通过相关基因的复制和缺失产生新功能的随机遗传网络。
Transforming growth factor-β (TGF-β) signaling is essential for numerous biologic functions. It is a highly conserved pathway found in all metazoans including the nematode Caenorhabditis elegans, which has also been pivotal in identifying many components. Utilizing a comparative evolutionary approach, we explored TGF-β signaling in nine nematode species and revealed striking variability in TGF-β gene frequency across the lineage. Of the species analyzed, gene duplications in the DAF-7 pathway appear common with the greatest disparity observed in Pristionchus pacificus. Specifically, multiple paralogues of daf-3, daf-4 and daf-7 were detected. To investigate this additional diversity, we induced mutations in 22 TGF-β components and generated corresponding double, triple, and quadruple mutants revealing both conservation and diversification in function. Although the DBL-1 pathway regulating body morphology appears highly conserved, the DAF-7 pathway exhibits functional divergence, notably in some aspects of dauer formation. Furthermore, the formation of the phenotypically plastic mouth in P. pacificus is partially influenced through TGF-β with the strongest effect in Ppa-tag-68. This appears important for numerous processes in P. pacificus but has no known function in C. elegans. Finally, we observe behavioral differences in TGF-β mutants including in chemosensation and the establishment of the P. pacificus kin-recognition signal. Thus, TGF-β signaling in nematodes represents a stochastic genetic network capable of generating novel functions through the duplication and deletion of associated genes.
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影响因子：
29
作者：
Greer, Elisabeth R.;Perez, Carissa L.;Van Gilst, Marc R.;Lee, Brian H.;Ashrafi, Kaveh
通讯作者：
Ashrafi, Kaveh
DOI：
10.1002/dvdy.423
发表时间：
2022-01
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Goodman MB;Savage-Dunn C
通讯作者：
Savage-Dunn C
DOI：
10.1534/g3.117.300416
发表时间：
2018-01-04
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Clark JF;Meade M;Ranepura G;Hall DH;Savage-Dunn C
通讯作者：
Savage-Dunn C
影响因子：
1.5
作者：
Crook, M;Thompson, FJ;Viney, ME
通讯作者：
Viney, ME
影响因子：
64.5
作者：
GEORGI, LL;ALBERT, PS;RIDDLE, DL
通讯作者：
RIDDLE, DL