A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.
A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.
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在诱导的原始生殖细胞规范和遗传程序之间对VASA调节中的保守节点。
DOI：
10.1016/j.ydbio.2021.11.007
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发表时间：
2022-03
影响因子：
2.7
通讯作者：
Wessel GM
中科院分区：
文献类型：
--
作者：
Perillo M;Swartz SZ;Wessel GM
原始生殖细胞（PGCs）在早期发育中由多种机制指定。在一些动物中，PGC是通过母体决定因素的遗传来指定的，而在另一些动物中，在被认为代表祖先模式的过程中，PGC命运是由细胞相互作用诱导的。虽然在特定的生殖细胞中表达的末端因子是广泛保守的，但调节这些因子的机制可以是多种多样的。在这里，我们表明，生殖细胞规格的翻译后机制是保守的两个棘皮动物物种认为采用不同的生殖系分离策略。海胆通过遗传机制早期分离其生殖系。DEAD盒RNA解旋酶Vasa是一种保守的生殖系因子，通过E3-泛素连接酶Gustavus在未来的体细胞中降解而在PGC中富集。这种翻译后活性发生在发育早期，基本上在原肠胚形成之前。我们在海星星星胚胎的生殖细胞特化中测试了这一过程，这一过程在原肠胚形成后决定PGC命运的信号传导机制。我们发现，Vasa-GFP蛋白成为局限于PGCs的海星星，即使注入的mRNA是整个胚胎。然而，Gustavus耗尽导致蛋白质的均匀积累。这些数据表明，Gustavus介导的Vasa营业额在体细胞中是保守的物种之间，否则不同的PGC规范机制。由于Gustavus最初在果蝇中被鉴定为在Vasa调节中具有类似的功能，因此我们得出结论，PGC形成中的Vasa调节的这个节点是祖先的，并且从祖先的诱导PGC规范程序到遗传的PGC规范机制在进化上是可转座的。
Primordial germ cells (PGCs) are specified by diverse mechanisms in early development. In some animals, PGCs are specified via inheritance of maternal determinants, while in others, in a process thought to represent the ancestral mode, PGC fate is induced by cell interactions. Although the terminal factors expressed in specified germ cells are widely conserved, the mechanisms by which these factors are regulated can be widely diverse. Here we show that a post-translational mechanism of germ cell specification is conserved between two echinoderm species thought to employ divergent germ line segregation strategies. Sea urchins segregate their germ line early by an inherited mechanism. The DEAD-box RNA - helicase Vasa, a conserved germline factor, becomes enriched in the PGCs by degradation in future somatic cells by the E3-ubiquitin-ligase Gustavus. This post-translational activity occurs early in development, substantially prior to gastrulation. Here we test this process in germ cell specification of sea star embryos, which tive signaling mechanisms after gastrulation for PGC fate determination. We find that Vasa-GFP protein becomes restricted to the PGCs in the sea star even though the injected mRNA is present throughout the embryo. Gustavus depletion, however, results in uniform accumulation of the protein. These data demonstrate that Gustavus-mediated Vasa turnover in somatic cells is conserved between species with otherwise divergent PGC specification mechanisms. Since Gustavus was originally identified in Drosophila melanogaster to have similar functions in Vasa regulation, we conclude that this node of Vasa regulation in PGC formation is ancestral and evolutionarily transposable from the ancestral, induced PGC specification program to an inherited PGC specification mechanism.
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影响因子：
14.9
作者：
Cameron RA;Samanta M;Yuan A;He D;Davidson E
通讯作者：
Davidson E
DOI：
10.1007/978-1-0716-0974-3_12
发表时间：
2021
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Perillo M;Paganos P;Spurrell M;Arnone MI;Wessel GM
通讯作者：
Wessel GM
DOI：
10.1093/database/bax074
发表时间：
2017-01-01
期刊：
Database : the journal of biological databases and curation
影响因子：
--
作者：
Kudtarkar P;Cameron RA
通讯作者：
Cameron RA
影响因子：
2.7
作者：
Gustafson EA;Yajima M;Juliano CE;Wessel GM
通讯作者：
Wessel GM
影响因子：
4.6
作者：
Fresques, Tara M.;Wessel, Gary M.
通讯作者：
Wessel, Gary M.