Titanium particle-challenged osteoblasts promote osteoclastogenesis and osteolysis in a murine model of periprosthestic osteolysis.
Titanium particle-challenged osteoblasts promote osteoclastogenesis and osteolysis in a murine model of periprosthestic osteolysis.
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DOI：
10.1016/j.actbio.2013.03.010
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发表时间：
2013-07
影响因子：
9.7
通讯作者：
Yang, Shang-You
中科院分区：
文献类型：
--
作者：
Jiang, Yunpeng;Jia, Tanghong;Gong, Weiming;Wooley, Paul H.;Yang, Shang-You
本研究研究了钛合金颗粒激发成骨骨髓基质细胞（BMSCs）和巨噬细胞谱系细胞在小鼠膝关节假体衰竭模型中的相互作用行为。从雄性BALB/c小鼠股骨中分离骨髓间充质干细胞，在成骨培养基中诱导。分离24小时后，在完全诱导培养基中，分别用1、3或5mg/ml钛颗粒刺激骨髓间充质干细胞7天。在第2、4和6天收集培养基，在第7天收获细胞进行碱性磷酸酶（ALP）测定/染色。MTT法定期测定Ti颗粒存在下的细胞增殖情况。植入钛针胫骨植入物的小鼠关节内注射50μl含有5×105 Ti颗粒挑战骨髓源性成骨细胞的培养基，术后2周重复注射。钛针植入的对照小鼠接受naïve成骨细胞输注。4周牺牲后，收集各组植入膝关节进行生物力学拔针试验、组织学评价和关节组织提取mRNA的RT-PCR分析。ti颗粒在高、低浓度下均能显著刺激骨髓间充质干细胞成骨细胞的增殖（p<0.05），颗粒剂量间无显著差异。在ti颗粒相互作用后，ALP的表达明显降低，其中高剂量颗粒组的差异有统计学意义（p<0.05）。此外，与接受naïve成骨细胞培养基的小鼠外周血单核细胞相比，将短期激发（48小时）成骨细胞收集的培养基添加到小鼠外周血单核细胞培养中，TRAP+细胞数量显著增加（p<0.05）。将成骨细胞引入小鼠假钉失败模型的关节内，导致假体界面剪切强度降低，假体周围软组织形成更厚，表明钛颗粒挑战的成骨细胞有助于假体周围骨溶解。对比成骨细胞注射后种植体周围组织样本的基因表达谱，各组间RunX2或Osterix/Sp7基因表达差异无统计学意义。MMP-2、IL-1、TNF-α、RANKL、TRAP基因表达在成骨损伤组明显升高（p<0.05）。综上所述，钛合金颗粒对骨髓成骨细胞祖细胞的生长、成熟和功能有干扰作用。颗粒挑战成骨细胞似乎表达调节破骨细胞发生和假体周围骨溶解的介质。
The current study investigates the interactive behavior of titanium alloy particle-challenged osteoblastic bone marrow stromal cells (BMSCs) and macrophage lineage cells in a murine knee-prosthesis failure model. BMSCs were isolated from male BALB/c mice femurs and induced in osteogenic medium. At 24 hours after isolation, BMSCs in complete induction medium were challenged with 1, 3, or 5mg/ml titanium particles for 7 days. Culture media were collected at 2, 4 and 6 days and cells were harvested at 7 days for alkaline phosphatase (ALP) assay/stains. Cell proliferation in the presence of Ti particles was periodically evaluated by MTT assay. Mice implanted with titanium-pin tibial implants were given an intra-articular injection of 50μl medium containing 5×105 Ti particles-challenged bone marrow derived osteoblastic cells, followed by a repeat injection at 2 weeks post-op. Control mice with titanium-pin implants received a naïve osteoblastic cell transfusion. After sacrifice at 4 week, the implanted knee joint of each group was collected for biomechanical pin-pullout testing, histological evaluation and RT-PCR analysis of mRNA extracted from the joint tissues. Ti-particles significantly stimulated the proliferation of BMSC-derived osteoblastic cells at both high and low particle concentrations (p<0.05), with no marked differences between the particle doses. ALP expression was diminished following Ti-particle interactions, especially in the high dose particle group (p<0.05). In addition, the culture media collected from short-term challenged (48 hours) osteoblasts significantly increased the numbers of TRAP+ cells when added to mouse peripheral blood monocytes cultures, in comparison with the monocytes cells receiving naïve osteoblasts media (p<0.05). Intra-articular introduction of the osteoblastic cells to the mouse pin-implant failure model resulted in reduced implant interfacial shear strength and thicker peri-implant soft-tissue formation, suggesting that titanium particles-challenged osteoblasts contributed to periprosthetic osteolysis. Comparison of the gene expression profiles among the peri-implant tissue samples following osteoblast injection did not find significant difference in RunX2 or Osterix/Sp7 between the groups. However, MMP-2, IL-1, TNF-α, RANKL, and TRAP gene expressions were elevated in the challenged-osteoblast group (p<0.05). In conclusion, titanium alloy particles were shown to interfere with the growth, maturation, and functions of the bone marrow osteoblast progenitor cells. Particle-challenged osteoblasts appear to express mediators that regulate osteoclastogenesis and peri-prosthetic osteolysis.
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影响因子：
14
作者：
Granchi, D;Amato, I;Giunti, A
通讯作者：
Giunti, A
DOI：
10.1097/01.blo.0000229365.57985.96
发表时间：
2006-12-01
影响因子：
4.2
作者：
Hundric-Haspl, Zeljka;Pecina, Marko;Jukic, Irena
通讯作者：
Jukic, Irena
影响因子：
3.8
作者：
Stea, S;Visentin, M;Toni, A
通讯作者：
Toni, A
影响因子：
2.8
作者：
Yang, Shang-You;Zhang, Kai;Wooley, Paul H.
通讯作者：
Wooley, Paul H.
影响因子：
2.8
作者：
Yang, Shang-You;Yu, Haiying;Wooley, Paul H.
通讯作者：
Wooley, Paul H.