Control of the olive fruit fly using genetics-enhanced sterile insect technique.
Control of the olive fruit fly using genetics-enhanced sterile insect technique.
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DOI：
10.1186/1741-7007-10-51
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发表时间：
2012-06-19
期刊：
影响因子：
5.4
通讯作者：
Alphey L
中科院分区：
文献类型：
--
作者：
Ant T;Koukidou M;Rempoulakis P;Gong HF;Economopoulos A;Vontas J;Alphey L
橄榄小实蝇是橄榄商品化生产的主要节肢动物害虫，对世界范围内的橄榄作物造成了广泛的危害。目前的控制技术依赖于喷洒化学杀虫剂。昆虫不育技术(SIT)提供了一种替代的、环境友好的和特定物种的种群控制方法。尽管SIT对其他粉虱类害虫的防治非常成功，但之前在橄榄蝇上进行的SIT试验结果令人失望。关键问题包括实验室饲养的昆虫的昼夜交配节律改变，导致野生和释放的不育种群之间的不同步交配活动，以及经辐射消毒的大规模饲养苍蝇的低竞争力。因此，产生有竞争力的、仅限雄性放飞的种群被认为是成功的橄榄坐蝇的必要前提。我们开发了一套有条件的雌性致死橄榄蝇品系(名为释放携带显性致死基因的昆虫；RIDL®)，提供高度渗透性的雌性特异性致死性、显性荧光标记和遗传不育。我们发现，铅品系OX3097D-Bol的雄性与野生橄榄蝇有很强的性竞争，2)与野生雌性表现出同步的交配活动，3)对野生雌性重新交配有适当的抵抗力。此外，我们通过一个大型的原理验证实验表明，每周将OX3097D-BOL雄性释放到笼养野生橄榄蝇的稳定种群中可以导致种群迅速崩溃和最终灭绝。观察到的交配特征强烈表明，基于释放OX3097D-BOL雄蝇的方法将克服之前橄榄蝇坐尝试中遇到的关键困难。尽管需要现场确认，但通过OX3097D-BOL雄释放对笼养野生橄榄蝇种群的抑制和消除的原则证明，针对雌性的RIDL方法是一种可行的橄榄蝇控制方法。我们的结论是，OX3097D-BOL的良好特性最终可能使基于SIT的有效控制橄榄蝇成为可能。
The olive fruit fly, Bactrocera oleae, is the major arthropod pest of commercial olive production, causing extensive damage to olive crops worldwide. Current control techniques rely on spraying of chemical insecticides. The sterile insect technique (SIT) presents an alternative, environmentally friendly and species-specific method of population control. Although SIT has been very successful against other tephritid pests, previous SIT trials on olive fly have produced disappointing results. Key problems included altered diurnal mating rhythms of the laboratory-reared insects, resulting in asynchronous mating activity between the wild and released sterile populations, and low competitiveness of the radiation-sterilised mass-reared flies. Consequently, the production of competitive, male-only release cohorts is considered an essential prerequisite for successful olive fly SIT. We developed a set of conditional female-lethal strains of olive fly (named Release of Insects carrying a Dominant Lethal; RIDL®), providing highly penetrant female-specific lethality, dominant fluorescent marking, and genetic sterility. We found that males of the lead strain, OX3097D-Bol, 1) are strongly sexually competitive with wild olive flies, 2) display synchronous mating activity with wild females, and 3) induce appropriate refractoriness to wild female re-mating. Furthermore, we showed, through a large proof-of-principle experiment, that weekly releases of OX3097D-Bol males into stable populations of caged wild-type olive fly could cause rapid population collapse and eventual eradication. The observed mating characteristics strongly suggest that an approach based on the release of OX3097D-Bol males will overcome the key difficulties encountered in previous olive fly SIT attempts. Although field confirmation is required, the proof-of-principle suppression and elimination of caged wild-type olive fly populations through OX3097D-Bol male releases provides evidence for the female-specific RIDL approach as a viable method of olive fly control. We conclude that the promising characteristics of OX3097D-Bol may finally enable effective SIT-based control of the olive fly.
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影响因子：
5.4
作者：
Phuc HK;Andreasen MH;Burton RS;Vass C;Epton MJ;Pape G;Fu G;Condon KC;Scaife S;Donnelly CA;Coleman PG;White-Cooper H;Alphey L
通讯作者：
Alphey L
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Fu, Guoliang;Condon, Kirsty C.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Gong, P;Epton, MJ;Alphey, L
通讯作者：
Alphey, L
影响因子：
2.6
作者：
Koukidou, M;Klinakis, A;Savakis, C
通讯作者：
Savakis, C