Viral regulatory region effects on vertical transmission of polyomavirus SV40 in hamsters.
Viral regulatory region effects on vertical transmission of polyomavirus SV40 in hamsters.
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DOI：
10.1016/j.virol.2008.12.040
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发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
Butel, Janet S.
中科院分区：
文献类型：
--
作者：
Patel, Niraj C.;Halvorson, Steven J.;Sroller, Vojtech;Arrington, Amy S.;Wong, Connie;Smith, E. O'Brian;Vilchez, Regis A.;Butel, Janet S.
病毒株差异影响多瘤病毒猿猴病毒40（SV 40）的致癌潜力。我们假设病毒株的差异也可能影响易感宿主中SV 40的垂直传播。妊娠的叙利亚金仓鼠腹腔内接种107个空斑形成单位的SV 40，并在分娩后（1-21天，6个月）处死后代。通过聚合酶链反应分析器官提取物中的SV 40 DNA。在半数以上的窝仔中检测到SV 40从母亲到后代的传播。大多数胎盘呈病毒阳性。接种含有复杂调控区的SV 40毒株的母亲比感染简单增强子病毒的母亲更容易传播病毒（p<0.001）。病毒在子代脑中的检出率高于脾脏（p<0.05）。几个后代在6个月龄时呈病毒阳性，表明病毒持续存在。到第21天，母体动物的几个组织中保留了病毒，并产生了T抗原抗体。这些结果表明，SV 40在仓鼠中复制，SV 40可以发生垂直传播，并且病毒调节区影响传播。
Viral strain differences influence the oncogenic potential of polyomavirus simian virus 40 (SV40). We hypothesized that viral strain differences might also affect vertical transmission of SV40 in susceptible hosts. Pregnant Syrian golden hamsters were inoculated intraperitoneally with 107 plaque-forming units of SV40 and offspring were sacrificed post-delivery (1-21 days, 6 mo). Organ extracts were analyzed for SV40 DNA by polymerase chain reaction assay. Transmission of SV40 from mother to offspring was detected in over half of litters. Most placentas were virus-positive. Mothers inoculated with SV40 strains containing complex regulatory regions transmitted virus more frequently than those infected with simple enhancer viruses (p<0.001). Virus was detected more often in progeny brain than in spleen (p<0.05). Several progeny were virus-positive at 6 months of age, suggesting viral persistence. Maternal animals retained virus in several tissues through day 21 and developed T-antigen antibodies. These results indicate that SV40 replicates in hamsters, vertical transmission of SV40 can occur, and the viral regulatory region influences transmission.
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