Microbial community composition of polyhydroxyalkanoate-accumulating organisms in full-scale wastewater treatment plants operated in fully aerobic mode.
Microbial community composition of polyhydroxyalkanoate-accumulating organisms in full-scale wastewater treatment plants operated in fully aerobic mode.
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DOI：
10.1264/jsme2.me12141
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发表时间：
2013
影响因子：
2.2
通讯作者：
Mino T
中科院分区：
文献类型：
--
作者：
Oshiki M;Onuki M;Satoh H;Mino T
生物降解有机物的去除是废水生物处理的重要目标之一。聚羟基烷酸酯（PHA）积累生物（PHAAOs）对生物降解有机物的去除有重要作用；然而，它们的微生物群落组成大多是未知的。在本研究中，采用荧光原位杂交（FISH）分析和FISH后尼罗河蓝A （NBA）染色技术，研究了8个全好氧模式下的污水处理厂（WWTPs）中PHAAOs的微生物群落组成。结果表明：1)PHAAOs占细胞总数的11% ~ 18%；2)8个全尺寸WWTPs中PHAAOs在菌域/门/类/目水平上的微生物群落组成相似，优势PHAAOs为Alphaproteobacteria和Betaproteobacteria类成员。通过16S rRNA基因克隆文库分析α-和β-变形菌PHAAOs的微生物群落组成，并进一步采用一套新设计的针对α-或β-变形菌PHAAOs 16S rRNA基因序列的寡核苷酸探针进行检测。结果表明，在Alphaproteobacteria和Betaproteobacteria中，PHAAOs的微生物群落组成不同，这可能导致了WWTPs之间PHA积累能力的差异（8.5-38.2 mg-C g-VSS−1 h−1）。本研究扩展了对全好氧模式下全规模污水处理厂中PHAAOs微生物多样性的认识。
The removal of biodegradable organic matter is one of the most important objectives in biological wastewater treatments. Polyhydroxyalkanoate (PHA)-accumulating organisms (PHAAOs) significantly contribute to the removal of biodegradable organic matter; however, their microbial community composition is mostly unknown. In the present study, the microbial community composition of PHAAOs was investigated at 8 full-scale wastewater treatment plants (WWTPs), operated in fully aerobic mode, by fluorescence in situ hybridization (FISH) analysis and post-FISH Nile blue A (NBA) staining techniques. Our results demonstrated that 1) PHAAOs were in the range of 11–18% in the total number of cells, and 2) the microbial community composition of PHAAOs was similar at the bacterial domain/phylum/class/order level among the 8 full-scale WWTPs, and dominant PHAAOs were members of the class Alphaproteobacteria and Betaproteobacteria. The microbial community composition of α- and β-proteobacterial PHAAOs was examined by 16S rRNA gene clone library analysis and further by applying a set of newly designed oligonucleotide probes targeting 16S rRNA gene sequences of α- or β-proteobacterial PHAAOs. The results demonstrated that the microbial community composition of PHAAOs differed in the class Alphaproteobacteria and Betaproteobacteria, which possibly resulted in a different PHA accumulation capacity among the WWTPs (8.5–38.2 mg-C g-VSS−1 h−1). The present study extended the knowledge of the microbial diversity of PHAAOs in full-scale WWTPs operated in fully aerobic mode.
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影响因子：
5
作者：
Gomez, JGC;Rodrigues, MFA;daSilva, LF
通讯作者：
daSilva, LF
影响因子：
4.4
作者：
KARR, DB;WATERS, JK;EMERICH, DW
通讯作者：
EMERICH, DW
影响因子：
12.8
作者：
Carucci, A;Dionisi, D;Smurra, P
通讯作者：
Smurra, P
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
2.2
作者：
Fukushima, Toshikazu;Uda, Naoki;Mino, Takashi
通讯作者：
Mino, Takashi