Indocyanine-type Infrared-820 Encapsulated Polymeric Nanoparticle-Assisted Photothermal Therapy of Cancer.
Indocyanine-type Infrared-820 Encapsulated Polymeric Nanoparticle-Assisted Photothermal Therapy of Cancer.
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DOI：
10.1021/acsomega.2c00306
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发表时间：
2022-04-12
期刊：
影响因子：
4.1
通讯作者：
Aryal, Santosh
中科院分区：
文献类型：
--
作者：
Marasini, Ramesh;Aryal, Santosh
有机小分子光敏剂具有良好的特性，以光响应治疗方式而闻名，包括光动力治疗。与传统的有机光敏剂光动力治疗中使用的紫外-可见（UV-vis）光相比，700 ~ 900 nm的近红外（NIR）光被血红蛋白、脂质和水等生物组织吸收和散射较少，因此，使用近红外激发可以大大增加穿透深度和发射。此外，近红外光的能量比紫外-可见光低，这可能是有益的，因为近红外光照射后组织中存在的荧光团的激活较少。然而，有机光敏剂的水稳定性低、分布非特异性、循环半衰期短等缺点限制了其在生物领域的广泛应用。近红外响应的小分子荧光剂是分子成像和热疗联合研究的重点。最近，一类新的近红外染料IR-820的激发和发射波长为710和820 nm，已被开发和探索，作为克服一些最常用的金纳米粒子光热治疗癌症的局限性的替代平台。本文以聚乙二醇偶联磷脂为外壳，聚乳酸乙醇酸（PLGA）为核心，合成了核-壳型生物相容性纳米载体包膜，包覆IR-820染料。采用纳米沉淀法制备了负载ir -820的纳米颗粒，并对其理化性质和光热效率进行了表征。这些纳米颗粒具有单分散特性，在生理pH下具有较高的稳定性，水动力粒径为103±8 nm，多分散指数为0.163±0.031。负载ir -820的纳米载体在黑暗中表现出良好的生物相容性，而在近红外激光激发下，对乳腺癌细胞（MCF-7）观察到显著的光毒性。因此，负载ir -820的磷脂模拟生物可降解脂质-聚合物复合纳米颗粒在癌症治疗方面具有很大的潜力。
Organic small-molecule photosensitizers are well-characterized and known for the light-responsive treatment modality including photodynamic therapy. Compared with ultraviolet–visible (UV–vis) light used in conventional photodynamic therapy with organic photosensitizers, near-infrared (NIR) light from 700 to 900 nm is less absorbed and scattered by biological tissue such as hemoglobin, lipids, and water, and thus, the use of NIR excitation can greatly increase the penetration depth and emission. Additionally, NIR light has lower energy than UV–vis that can be beneficial due to less activation of fluorophores present in tissues upon NIR irradiation. However, the low water stability, nonspecific distribution, and short circulation half-life of the organic photosensitizers limit its broad biological application. NIR responsive small-molecule fluorescent agents are the focus of extensive research for combined molecular imaging and hyperthermia. Recently a new class of NIR dye, IR-820 with excitation and emission wavelengths of 710 and 820 nm, has been developed and explored as an alternative platform to overcome some of the limitations of the most commonly used gold nanoparticles for photothermal therapy of cancer. Herein, we synthesized a core–shell biocompatible nanocarrier envelope made up of a phospholipid conjugated with poly(ethylene glycol) as a shell, while poly(lactic glycolic acid) (PLGA) was used as a core to encapsulate IR-820 dye. The IR-820-loaded nanoparticles were prepared by nanoprecipitation and characterized for their physicochemical properties and photothermal efficiency. These nanoparticles were monodispersed and highly stable in physiological pH with the hydrodynamic size of 103 ± 8 nm and polydispersity index of 0.163 ± 0.031. The IR-820-loaded nanocarrier showed excellent biocompatibility in the dark, whereas remarkable phototoxicity was observed with breast cancer cells (MCF-7) upon NIR laser excitation. Therefore, the IR-820-loaded phospholipid mimicking biodegradable lipid-polymer composite nanoparticles could have great potential for cancer theranostics.
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