Quantitative determination of the electron beam radiation dose for SARS-CoV-2 inactivation to decontaminate frozen food packaging.
Quantitative determination of the electron beam radiation dose for SARS-CoV-2 inactivation to decontaminate frozen food packaging.
复制标题
定量测定用于灭活 SARS-CoV-2 的电子束辐射剂量以净化冷冻食品包装
DOI：
10.1016/j.virs.2022.10.007
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发表时间：
2022-12
影响因子：
5.5
通讯作者：
Meng, Songdong
中科院分区：
文献类型：
--
作者：
Wang, Zihao;Liang, Zhentao;Wei, Rongguo;Wang, Hongwei;Cheng, Fang;Liu, Yang;Meng, Songdong
关键词：
在当前全球大流行期间，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）从冷链食品传播至一线工作人员，对公众健康构成了严重威胁。迫切需要设计出简洁的方法，以便在不同物理化学条件下有效灭活病毒，降低因冷链食品被病毒污染的表面而导致的传染风险。通过在冷链温度下，对高滴度的SARS-CoV-2进行不同时长的电子束照射，结果表明，2千戈瑞（kGy）的辐射剂量可将病毒滴度从104.5 半数组织培养感染剂量（TCID50）/毫升降至0。接下来，以人冠状病毒OC43（HCoV-OC43）作为合适的SARS-CoV-2替代病毒，确定3 kGy的高能电子辐射为在受试包装材料上使病毒滴度降低超过4个对数单位的灭活剂量。此外，采用定量逆转录聚合酶链反应（RT-qPCR）检测E、N和ORF1ab这三个病毒基因。在检测SARS-CoV-2时，TCID50与RT-qPCR之间存在很强的相关性。然而，RT-qPCR无法区分经辐射灭活的病毒与未辐照对照病毒的感染性。由于确定的有效病毒灭活辐射剂量远低于食品加工的安全剂量上限，我们的研究结果为设计基于辐射的方法来对冷冻食品中的SARS-CoV-2进行去污处理提供了依据。我们进一步证明，基于细胞的病毒检测对于评估去污策略对SARS-CoV-2的灭活效率至关重要。
电子束辐照是冷链条件下灭活SARS-CoV-2的有效方法。
有效灭活剂量确定为3 kGy，远低于食品加工的安全限值。
基于细胞的病毒检测对于评估去污策略对SARS-CoV-2的灭活效率至关重要。
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from cold-chain foods to frontline workers poses a serious public health threat during the current global pandemic. There is an urgent need to design concise approaches for effective virus inactivation under different physicochemical conditions to reduce the risk of contagion through viral contaminated surfaces of cold-chain foods. By employing a time course of electron beam exposure to a high titer of SARS-CoV-2 at cold-chain temperatures, a radiation dose of 2 kGy was demonstrated to reduce the viral titer from 104.5 to 0 median tissue culture infectious dose (TCID50)/mL. Next, using human coronavirus OC43 (HCoV-OC43) as a suitable SARS-CoV-2 surrogate, 3 kGy of high-energy electron radiation was defined as the inactivation dose for a titer reduction of more than 4 log units on tested packaging materials. Furthermore, quantitative reverse transcription PCR (RT-qPCR) was used to test three viral genes, namely, E, N, and ORF1ab. There was a strong correlation between TCID50 and RT-qPCR for SARS-CoV-2 detection. However, RT-qPCR could not differentiate between the infectivity of the radiation-inactivated and nonirradiated control viruses. As the defined radiation dose for effective viral inactivation fell far below the upper safe dose limit for food processing, our results provide a basis for designing radiation-based approaches for the decontamination of SARS-CoV-2 in frozen food products. We further demonstrate that cell-based virus assays are essential to evaluate the SARS-CoV-2 inactivation efficiency for the decontaminating strategies. Electron beam irradiation is an effective approach for SARS-CoV-2 inactivation under cold-chain conditions. The effective inactivation dose was defined as 3 kGy that is far below the safe limit for food processing. Cell-based virus assays are essential to evaluate the SARS-CoV-2 inactivation efficiency for the decontaminating strategies.
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DOI：
10.3390/v13030408
发表时间：
2021-03-05
期刊：
Viruses
影响因子：
--
作者：
Gidari A;Sabbatini S;Bastianelli S;Pierucci S;Busti C;Bartolini D;Stabile AM;Monari C;Galli F;Rende M;Cruciani G;Francisci D
通讯作者：
Francisci D
影响因子：
9.4
作者：
ELLIOTT, LH;MCCORMICK, JB;JOHNSON, KM
通讯作者：
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影响因子：
2.8
作者：
Brahmakshatriya, V.;Lupiani, B.;Reddy, S. M.
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影响因子：
4.6
作者：
Al-Hadyan K;Alsbeih G;Al-Harbi N;Judia SB;Al-Ghamdi M;Almousa A;Alsharif I;Bakheet R;Al-Romaih K;Al-Mozaini M;Al-Ghamdi S;Moftah B;Alhmaid R
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通过加热和紫外线照射从谱系B.1.1.7（Alpha），p.1（伽马）和B.1.110的SARS-COV-2分离株失活。
影响因子：
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作者：
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