Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection.
Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection.
复制标题
伽马射线对过滤面部呼吸器和SARS-COV-2检测的影响。
DOI：
10.1038/s41598-021-99414-6
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发表时间：
2021-10-06
影响因子：
4.6
通讯作者：
Alhmaid R
中科院分区：
文献类型：
--
作者：
Al-Hadyan K;Alsbeih G;Al-Harbi N;Judia SB;Al-Ghamdi M;Almousa A;Alsharif I;Bakheet R;Al-Romaih K;Al-Mozaini M;Al-Ghamdi S;Moftah B;Alhmaid R
为解决新冠肺炎(CoronaVirus)大流行期间滤面呼吸器(FFR)短缺的问题，医疗机构在应用了不同的净化方法(包括伽马辐射(GIR))后，被迫重复使用滤面呼吸器。本研究的目的是评价GIR对FFR过滤效率(FE)和SARS-CoV-2检测的影响。在典型剂量率(1.65kGyh)和低剂量率(0.5088 kGyh)下，对两种FFR类型(KN95型和N95-3型FFR面罩)在不同粒度(0.3-5kGy0-15kGy0-15kGy射线)照射后的FE进行了评估。用RT-qPCR方法检测GIR(0~50kGy)后SARS-CoV-2RNA基因(E基因和RdRp4基因)。随着GIR剂量的增加，两种口罩的FE总体上都有显著的降低(P <； 0.001)。FE上的GIR剂量率差异无统计学意义。GIR显示这两个基因的周期阈值( ≤ Ct)显著升高(P<0.001)，高剂量后未检测到。综上所述，高GIR(≥ 30kGy)可以完全降解SARS-CoV-2RNA，这表明其在FFR去污方面具有潜在的应用前景。然而，GIR以剂量和颗粒大小依赖的方式对FE产生不利影响，使其用于去污FFR存在争议。
To cope with the shortage of filtering facepiece respirators (FFRs) during the coronavirus (COVID-19) pandemic, healthcare institutions were forced to reuse FFRs after applying different decontamination methods including gamma-irradiation (GIR). The aim of this study was to evaluate the effect of GIR on the filtration efficiency (FE) of FFRs and on SARS-CoV-2 detection. The FE of 2 FFRs types (KN95 and N95-3 M masks) was assessed at different particle sizes (0.3–5 µm) following GIR (0–15 kGy) delivered at either typical (1.65 kGy/h) or low (0.5088 kGy/h) dose rates. The detection of two SARS-CoV-2 RNA genes (E and RdRp4) following GIR (0–50 kGy) was carried out using RT-qPCR assay. Both masks showed an overall significant (P < 0.001) reduction in FE with increased GIR doses. No significant differences were observed between GIR dose rates on FE. The GIR exhibited significant increases (P ≤ 0.001) in the cycle threshold values (ΔCt) of both genes, with no detection following high doses. In conclusion, complete degradation of SARS-CoV-2 RNA can be achieved by high GIR (≥ 30 kGy), suggesting its potential use in FFRs decontamination. However, GIR exhibited adverse effects on FE in dose- and particle size-dependent manners, rendering its use to decontaminate FFRs debatable.
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影响因子：
3.1
作者：
Darnell ME;Subbarao K;Feinstone SM;Taylor DR
通讯作者：
Taylor DR
DOI：
10.3390/ijerph17176117
发表时间：
2020-08-22
影响因子：
--
作者：
Derraik JGB;Anderson WA;Connelly EA;Anderson YC
通讯作者：
Anderson YC
影响因子：
3.3
作者：
Feldmann, Friederike;Shupert, W. Lesley;Feldmann, Heinz
通讯作者：
Feldmann, Heinz
影响因子：
9.4
作者：
ELLIOTT, LH;MCCORMICK, JB;JOHNSON, KM
通讯作者：
JOHNSON, KM
影响因子：
2.9
作者：
Craven, E.;Hasanain, F.;Winters, M.
通讯作者：
Winters, M.