Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells.
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells.
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DOI：
10.3791/56331
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发表时间：
2017-11-17
期刊：
影响因子：
--
通讯作者：
Wende K
中科院分区：
文献类型：
--
作者：
Bekeschus S;Schmidt A;Niessner F;Gerling T;Weltmann KD;Wende K
在等离子体医学中，将温度接近脊椎动物系统温度的电离气体应用于细胞和组织。冷等离子体产生已知用于氧化还原调节健康和疾病中的生物过程的活性物质。临床前和临床证据表明等离子体治疗对慢性皮肤溃疡的愈合具有有益作用。其他新兴主题，如血浆癌症治疗，正在受到越来越多的关注。等离子体医学研究需要物理、化学和生物医学的跨学科专业知识。等离子体研究的一个目标是在各种特定应用中表征等离子体处理的细胞。这包括例如细胞计数和活力、细胞氧化、线粒体活性、细胞毒性和细胞死亡模式、细胞周期分析、细胞表面标志物表达和细胞因子释放。本研究描述了血浆生物医学研究所需的基本设备和工作流程。它描述了大气压氩等离子体射流的正确操作，特别是监测其基本发射光谱和进料气体设置，以调节活性物质的输出。使用高精度xyz表和计算机软件，射流以毫秒精度悬停在96孔板的腔体上，以微米精度获得最大的再现性。显示了用于氧化还原活性分子的液体分析的下游测定，并且靶细胞被等离子体处理。具体地，在不同连续测定的有效序列中但使用相同的细胞分析黑素瘤细胞：测量代谢活性、总细胞面积和钙网蛋白的表面标志物表达，钙网蛋白是对癌细胞的免疫原性细胞死亡重要的分子。这些测定从单个板中检索关于等离子体效应的内容丰富的生物信息。总之，这项研究描述了血浆医学研究的基本步骤和协议。
In plasma medicine, ionized gases with temperatures close to that of vertebrate systems are applied to cells and tissues. Cold plasmas generate reactive species known to redox regulate biological processes in health and disease. Pre-clinical and clinical evidence points to beneficial effects of plasma treatment in the healing of chronic ulcer of the skin. Other emerging topics, such as plasma cancer treatment, are receiving increasing attention. Plasma medical research requires interdisciplinary expertise in physics, chemistry, and biomedicine. One goal of plasma research is to characterize plasma-treated cells in a variety of specific applications. This includes, for example, cell count and viability, cellular oxidation, mitochondrial activity, cytotoxicity and mode of cell death, cell cycle analysis, cell surface marker expression, and cytokine release. This study describes the essential equipment and workflows required for such research in plasma biomedicine. It describes the proper operation of an atmospheric pressure argon plasma jet, specifically monitoring its basic emission spectra and feed gas settings to modulate reactive species output. Using a high-precision xyz-table and computer software, the jet is hovered in millisecond-precision over the cavities of 96-well plates in micrometer-precision for maximal reproducibility. Downstream assays for liquid analysis of redox-active molecules are shown, and target cells are plasma-treated. Specifically, melanoma cells are analyzed in an efficient sequence of different consecutive assays but using the same cells: measurement of metabolic activity, total cell area, and surface marker expression of calreticulin, a molecule important for the immunogenic cell death of cancer cells. These assays retrieve content-rich biological information about plasma effects from a single plate. Altogether, this study describes the essential steps and protocols for plasma medical research.
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影响因子：
--
作者：
Acharya A;Das I;Chandhok D;Saha T
通讯作者：
Saha T
影响因子：
4.6
作者：
Mirpour S;Piroozmand S;Soleimani N;Jalali Faharani N;Ghomi H;Fotovat Eskandari H;Sharifi AM;Mirpour S;Eftekhari M;Nikkhah M
通讯作者：
Nikkhah M
影响因子：
3.7
作者：
Kluge S;Bekeschus S;Bender C;Benkhai H;Sckell A;Below H;Stope MB;Kramer A
通讯作者：
Kramer A
H2O2和NO2在寒冷大气中血浆引起的细胞死亡中的协同作用。
影响因子：
4.6
作者：
Girard PM;Arbabian A;Fleury M;Bauville G;Puech V;Dutreix M;Sousa JS
通讯作者：
Sousa JS
影响因子：
3.5
作者：
Gherardi, Matteo;Turrini, Eleonora;Fimognari, Carmela
通讯作者：
Fimognari, Carmela