In Vivo Molecular K-Edge Imaging of Atherosclerotic Plaque Using Photon-counting CT.
In Vivo Molecular K-Edge Imaging of Atherosclerotic Plaque Using Photon-counting CT.
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DOI：
10.1148/radiol.2021203968
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发表时间：
2021-07
期刊：
影响因子：
19.7
通讯作者：
Douek PC
中科院分区：
文献类型：
--
作者：
Si-Mohamed SA;Sigovan M;Hsu JC;Tatard-Leitman V;Chalabreysse L;Naha PC;Garrivier T;Dessouky R;Carnaru M;Boussel L;Cormode DP;Douek PC
巨噬细胞负荷是动脉粥样硬化斑块破裂风险的一个主要因素，其评价仍然具有挑战性的分子非侵入性成像方法。具有k边缘成像的光子计数CT（PCCT）旨在允许使用金纳米颗粒特异性检测巨噬细胞。结合金纳米颗粒进行k边缘成像，以检测和定量兔动脉粥样硬化动脉瘤内的巨噬细胞负荷。动脉粥样硬化和对照新西兰白色兔在静脉注射金纳米颗粒（3.5 mL/kg，65 mg金/ml）之前和之后2天的几个时间点成像。随访结束时，通过静脉注射碘化造影剂进行主动脉CT血管造影。重建金k边缘和常规CT图像，用于巨噬细胞负荷的定性和定量评估。将PCCT成像结果与组织学检查、定量组织形态计量学、透射电子显微镜和定量电感耦合等离子体发射光谱法的结果进行比较。计算免疫染色切片中测量的巨噬细胞面积与相应PCCT切片中测量的金浓度和衰减之间的Pearson相关性。对7只动脉粥样硬化家兔和4只无动脉粥样硬化对照家兔进行了分析。在动脉粥样硬化家兔中，在注射前观察到沿着主动脉壁的钙化。在注射金纳米颗粒后2天，仅金k边缘图像允许区分钙化内的斑块增强和血管造影期间的管腔增强。在35个斑块（每只兔5个）中，在壁内测量的金浓度和巨噬细胞面积之间观察到良好的相关性（r = 0.82; 95%CI：0.67，0.91; P < .001），这高于在常规CT图像上观察到的相关性（r = 0.41; 95%CI：0.09，0.65; P = .01）。透射电子显微镜和电感耦合等离子体发射光谱分析证实了金k边成像结果。光子计数CT与金纳米粒子允许无创性评估的分子和解剖信息在体内兔动脉粥样硬化斑块。在CC BY 4.0许可下发布。在线补充材料可用于本文。另见Leiner在本期的社论。
Macrophage burden is a major factor in the risk of atherosclerotic plaque rupture, and its evaluation remains challenging with molecular noninvasive imaging approaches. Photon-counting CT (PCCT) with k-edge imaging aims to allow for the specific detection of macrophages using gold nanoparticles. To perform k-edge imaging in combination with gold nanoparticles to detect and quantify the macrophage burden within the atherosclerotic aortas of rabbits. Atherosclerotic and control New Zealand white rabbits were imaged before and at several time points up to 2 days after intravenous injection of gold nanoparticles (3.5 mL/kg, 65 mg gold per milliliter). Aortic CT angiography was performed at the end of the follow-up using an intravenous injection of an iodinated contrast material. Gold k-edge and conventional CT images were reconstructed for qualitative and quantitative assessment of the macrophage burden. PCCT imaging results were compared with findings at histologic examination, quantitative histomorphometry, transmission electron microscopy, and quantitative inductively coupled plasma optical emission spectrometry. Pearson correlations between the macrophage area measured in immunostained sections and the concentration of gold and attenuation measured in the corresponding PCCT sections were calculated. Seven rabbits with atherosclerosis and four control rabbits without atherosclerosis were analyzed. In atherosclerotic rabbits, calcifications were observed along the aortic wall before injection. At 2 days after injection of gold nanoparticles, only gold k-edge images allowed for the distinction of plaque enhancement within calcifications and for lumen enhancement during angiography. A good correlation was observed between the gold concentration measured within the wall and the macrophage area in 35 plaques (five per rabbit) (r = 0.82; 95% CI: 0.67, 0.91; P < .001), which was higher than that observed on conventional CT images (r = 0.41; 95% CI: 0.09, 0.65; P = .01). Transmission electron microscopy and inductively coupled plasma optical emission spectrometry analyses confirmed the gold k-edge imaging findings. Photon-counting CT with gold nanoparticles allowed for the noninvasive evaluation of both molecular and anatomic information in vivo in rabbits with atherosclerotic plaques. Published under a CC BY 4.0 license. Online supplemental material is available for this article. See also the editorial by Leiner in this issue.
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影响因子：
4.6
作者：
Cormode DP;Si-Mohamed S;Bar-Ness D;Sigovan M;Naha PC;Balegamire J;Lavenne F;Coulon P;Roessl E;Bartels M;Rokni M;Blevis I;Boussel L;Douek P
通讯作者：
Douek P
影响因子：
4.6
作者：
Kim J;Bar-Ness D;Si-Mohamed S;Coulon P;Blevis I;Douek P;Cormode DP
通讯作者：
Cormode DP
影响因子：
17.1
作者：
Lobatto, Mark E.;Calcagno, Claudia;Millon, Antoine;Senders, Max L.;Fay, Francois;Robson, Philip M.;Ramachandran, Sarayu;Binderup, Tina;Paridaans, Maarten P. M.;Sensarn, Steven;Rogalla, Stephan;Gordon, Ronald E.;Cardoso, Luis;Storm, Gert;Metselaar, Josbert M.;Contag, Christopher H.;Stroes, Erik S. G.;Fayad, Zahi A.;Mulder, Willem J. M.
通讯作者：
Mulder, Willem J. M.
影响因子：
6.7
作者：
Si-Mohamed S;Cormode DP;Bar-Ness D;Sigovan M;Naha PC;Langlois JB;Chalabreysse L;Coulon P;Blevis I;Roessl E;Erhard K;Boussel L;Douek P
通讯作者：
Douek P
影响因子：
4.6
作者：
Halttunen, Niki;Lerouge, Frederic;Parola, Stephane
通讯作者：
Parola, Stephane