Tensile properties, collagen content, and crosslinks in connective tissues of the immature knee joint.
Tensile properties, collagen content, and crosslinks in connective tissues of the immature knee joint.
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DOI：
10.1371/journal.pone.0026178
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Eleswarapu SV;Responte DJ;Athanasiou KA
膝关节的主要结缔组织在运动过程中协同作用，以提供关节稳定性、平滑的关节连接、减震和机械应力的分布。这些功能在很大程度上是由组织的内在物质特性赋予的，而这些内在物质特性又由生物化学组成决定。深入了解膝关节结缔组织的结构-功能关系，为组织工程提供设计参数。本研究的目的是对未成熟小牛的髁状软骨、髌骨软骨、内侧和外侧韧带、头侧和尾侧交叉韧带（分别类似于人类的前交叉韧带和后交叉韧带）、内侧和外侧副韧带以及髌骨韧带的拉伸性能、胶原含量和吡啶啉交联丰度进行全面表征。拉伸刚度和强度最大的髌骨和髌韧带，最低的透明软骨和交叉韧带，这些拉伸结果反映了胶原蛋白含量的趋势。尽管关节相对不成熟，但在每个组织中均发现吡啶啉交联，并且在组织之间观察到显著差异。值得注意的是，对于交叉韧带和髌韧带，交联密度在确定拉伸刚度方面似乎比胶原蛋白含量更重要。据我们所知，这项研究是第一个检查拉伸性能，胶原蛋白含量和吡啶啉交联丰度在直接头对头比较所有主要结缔组织的膝盖。这也是第一项报告吡啶啉交联密度结果的研究，表明其在确定某些组织的拉伸刚度方面优于胶原蛋白。
The major connective tissues of the knee joint act in concert during locomotion to provide joint stability, smooth articulation, shock absorption, and distribution of mechanical stresses. These functions are largely conferred by the intrinsic material properties of the tissues, which are in turn determined by biochemical composition. A thorough understanding of the structure-function relationships of the connective tissues of the knee joint is needed to provide design parameters for efforts in tissue engineering. The objective of this study was to perform a comprehensive characterization of the tensile properties, collagen content, and pyridinoline crosslink abundance of condylar cartilage, patellar cartilage, medial and lateral menisci, cranial and caudal cruciate ligaments (analogous to anterior and posterior cruciate ligaments in humans, respectively), medial and lateral collateral ligaments, and patellar ligament from immature bovine calves. Tensile stiffness and strength were greatest in the menisci and patellar ligament, and lowest in the hyaline cartilages and cruciate ligaments; these tensile results reflected trends in collagen content. Pyridinoline crosslinks were found in every tissue despite the relative immaturity of the joints, and significant differences were observed among tissues. Notably, for the cruciate ligaments and patellar ligament, crosslink density appeared more important in determining tensile stiffness than collagen content. To our knowledge, this study is the first to examine tensile properties, collagen content, and pyridinoline crosslink abundance in a direct head-to-head comparison among all of the major connective tissues of the knee. This is also the first study to report results for pyridinoline crosslink density that suggest its preferential role over collagen in determining tensile stiffness for certain tissues.
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影响因子：
--
作者：
Hootman, JM;Helmick, CG
通讯作者：
Helmick, CG
影响因子：
3.8
作者：
Hoben, Gwendolyn A.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
DOI：
10.1002/jbm.820290915
发表时间：
1995-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
CHU, CR;COUTTS, RD;AMIEL, D
通讯作者：
AMIEL, D
DOI：
10.1115/1.429656
发表时间：
2000-06-01
影响因子：
1.7
作者：
Mauck, RL;Soltz, MA;Ateshian, GA
通讯作者：
Ateshian, GA
DOI：
10.2519/jospt.1998.28.4.192
发表时间：
1998-10-01
影响因子：
6.1
作者：
Buckwalter, JA
通讯作者：
Buckwalter, JA