Chemiluminescence resonance energy transfer-based detection for microchip electrophoresis.
Chemiluminescence resonance energy transfer-based detection for microchip electrophoresis.
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DOI：
10.1021/ac9027643
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发表时间：
2010-03-01
影响因子：
7.4
通讯作者：
Liu, Yi-Ming
中科院分区：
文献类型：
--
作者：
Zhao, Shulin;Huang, Yong;Shi, Ming;Liu, Rongjun;Liu, Yi-Ming
由于微纳流体器件中的通道非常小，因此需要在微芯片电泳(MCE)之后进行灵敏的检测。这项工作描述了一种基于化学发光共振能量转移(CRET)的高灵敏但通用的MCE检测方案。研究发现，在鲁米诺-NaBRO-QD体系中，鲁米诺供体和量子点(QD)受体之间存在有效的CRET，并被生物胺和硫醇、氨基酸、有机酸和类固醇等某些具有生物意义的有机化合物灵敏地抑制。这些发现使得对被测化合物开发灵敏的MCE-CL分析成为可能。所提出的MCE-CL方法显示出理想的分析品质因数，如较宽的线性响应浓度范围。对多巴胺和肾上腺素等生物胺的检出限为~10−9M，对生物硫醇(如谷胱甘肽和乙酰半胱氨酸)、有机酸(如抗坏血酸和尿酸)、雌激素和天然氨基酸的检出限为~10−8M。这些方法的灵敏度是以前报道的基于化学发光、电化学或激光诱导荧光检测的MCE方法的10到1000倍。为了评价目前的MCE-CL方法在实际生物样品分析中的适用性，将其用于单个人红细胞中氨基酸的测定。共检测到赖氨酸、丝氨酸、丙氨酸、谷氨酸、色氨酸等9种氨基酸。含量在3~31amol/cell之间。该方法简便、快速、重现性好、灵敏度高。
Since the channels in micro- and nanofluidic devices are extremely small, a sensitive detection is required following microchip electrophoresis (MCE). This work describes a highly sensitive and yet universal detection scheme based on chemiluminescence resonance energy transfer (CRET) for MCE. It was found that an efficient CRET occurred between a luminol donor and a CdTe quantum dot (QD) acceptor in the luminol-NaBrO-QD system, and that it was sensitively suppressed by the presence of certain organic compounds of biological interest including biogenic amines and thiols, amino acids, organic acids, and steroids. These findings allowed developing sensitive MCE-CL assays for the tested compounds. The proposed MCE-CL methods showed desired analytical figures of merit such as a wide concentration range of linear response. Detection limits obtained were ~10−9 M for biogenic amines including dopamine and epinephrine, and ~ 10−8 M for biogenic thiols (e.g. glutathione and acetylcysteine), organic acids (i.e. ascorbic acid and uric acid), estrogens, and native amino acids. These were 10 to 1000 times more sensitive than those of previously reported MCE-based methods with chemiluminescence, electrochemical, or laser induced fluorescence detection for quantifying corresponding compounds. To evaluate the applicability of the present MCE-CL method for analyzing real biological samples, it was used to determine amino acids in individual human red blood cells. Nine amino acids including Lys, Ser, Ala, Glu, Trp, etc. were detected. The contents ranged from 3 to 31 amol /cell. The assay proved to be simple, quick, reproducible, and very sensitive.
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影响因子：
6.1
作者：
Su, RG;Lin, JM;Yamada, M
通讯作者：
Yamada, M
影响因子：
7.4
作者：
Jacobson, SC;Ramsey, JM
通讯作者：
Ramsey, JM
影响因子：
29.4
作者：
Li, J;Hong, X;Li, TJ
通讯作者：
Li, TJ
影响因子：
7.4
作者：
Liu, BF;Ozaki, M;Terabe, S
通讯作者：
Terabe, S
DOI：
10.1016/j.jchromb.2008.05.035
发表时间：
2008-07-01
影响因子：
3
作者：
Sun, Xuemei;Niu, Yan;Zhang, Shusheng
通讯作者：
Zhang, Shusheng