Zebrafish (Danio rerio) responds to the animated image of a predator: towards the development of an automated aversive task.
Zebrafish (Danio rerio) responds to the animated image of a predator: towards the development of an automated aversive task.
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DOI：
10.1016/j.bbr.2009.03.003
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发表时间：
2009-08-12
影响因子：
2.7
通讯作者：
Pereira, Terence
中科院分区：
文献类型：
--
作者：
Gerlai, Robert;Fernandes, Yohaan;Pereira, Terence
斑马鱼在基本的大脑行为研究、行为遗传学和转化研究中越来越受欢迎，因为它提供了一种廉价而有效的啮齿动物替代品。异常夸大的恐惧和焦虑是人类社会中最普遍的神经精神疾病，其机制尚不清楚。这些疾病仍然是未得到满足的主要医疗需求。行为学上相关的恐惧范式涉及到捕食者的呈现，或捕食者的刺激特征，已被提出用于恐惧反应的建模和分析。以前，我们已经证明斑马鱼对同域捕食者印度叶鱼的视觉刺激有特异性反应。在当前的论文中，我们展示了这种捕食者的动画（移动）图像引发了显著的行为反应，这些反应可以通过视频跟踪来量化。由于刺激呈现和行为反应量化都是计算机化和自动化的，我们认为该范式适用于高通量筛选，一旦药理学和行为学验证，可能在检测化合物的抗焦虑或致焦虑特性或识别改变恐惧的突变方面具有实用价值。
Zebrafish is gaining popularity in basic behavioral brain research, behavior genetics, and in translational studies because it offers a cheap and efficient alternative to rodents. Abnormally exaggerated fear and anxiety are some of the most prevalent neuropsychiatric diseases in the human society whose mechanisms are not well understood. These diseases still represent major unmet medical needs. Ethologically relevant fear paradigms involving the presentation of predators, or of stimuli characteristic of predators, have been proposed as appropriate for the modeling and the analysis of fear responses. Previously, we have shown that zebrafish respond specifically to visual stimuli of their sympatric predator, the Indian leaf fish. In the current paper we show that an animated (moving) image of this predator elicits significant behavioral responses and that these responses can be quantified using video-tracking. As stimulus presentation and behavioral response quantification are both computerized and automated, we suggest the paradigm is appropriate for high throughput screening and, once pharmacologically and behaviorally validated, may have utility in the detection of anxiolytic or anxiogenic properties of compounds or in the identification of fear altering mutations.
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影响因子：
2.7
作者：
Miller, Noam;Gerlai, Robert
通讯作者：
Gerlai, Robert
影响因子：
8.2
作者：
Apfelbach, R;Blanchard, CD;McGregor, IS
通讯作者：
McGregor, IS
DOI：
10.1016/j.pnpbp.2003.09.012
发表时间：
2003-12-01
影响因子：
5.6
作者：
Blanchard, DC;Griebel, G;Blanchard, RJ
通讯作者：
Blanchard, RJ
影响因子：
8.2
作者：
Eilam, D
通讯作者：
Eilam, D
影响因子：
2.7
作者：
Miller, Noam Y.;Gerlai, Robert
通讯作者：
Gerlai, Robert