Nucleus ambiguus cholinergic neurons activated by acupuncture: relation to enkephalin.
Nucleus ambiguus cholinergic neurons activated by acupuncture: relation to enkephalin.
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DOI：
10.1016/j.brainres.2012.01.006
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发表时间：
2012-03-09
期刊：
影响因子：
2.9
通讯作者：
Longhurst JC
中科院分区：
文献类型：
--
作者：
Guo ZL;Li M;Longhurst JC
针灸调节植物神经功能。我们之前的研究表明，电针（EA）尖石内关穴（P5-P6，位于正中神经下方）可部分通过阿片类药物机制抑制中枢交感神经流出并减弱兴奋性心血管反射。目前尚不清楚这些穴位的电针是否影响副交感系统。因此，利用 c-Fos 表达，我们检查了 EA 对疑核 (NAmb) 神经元的激活、它们与胆碱能（节前副交感神经）神经元和含有脑啡肽的神经元的关系。为了增强对含有脑啡肽的细胞体的检测，在 EA 前 30 小时将秋水仙碱 (90-100 μg/kg) 注射到猫的蛛网膜下腔中，或进行 EA 的假手术对照。双侧压力神经支配和颈部迷走神经切断术后，在 P5-P6 穴位进行 30 分钟 EA 或对照刺激（在 P5-P6 处进针，无刺激）。虽然在一些髓质核（例如中缝）中观察到含有脑啡肽的核周体，但在 NAmb 中仅发现含有脑啡肽的神经元突起。与对照组 (n=4) 相比，在 EA 处理的猫的 NAmb 中发现了更多的 c-Fos 免疫反应性，主要位于含有脑啡肽的纤维附近 (n=5；P<0.01)。此外，在 EA 处理的动物中鉴定出 NAmb 中用 c-Fos 和胆碱乙酰转移酶双重标记的神经元，但在对照动物中未鉴定到。这些数据首次证明 EA 激活 NAmb 中的节前副交感神经元。由于它们距离很近，这些 EA 激活的神经元可能与含有脑啡肽的神经纤维相互作用。这些结果表明，P5-P6 穴位的电针有可能通过脑啡肽能机制影响副交感神经流出和心血管功能。
Acupuncture regulates autonomic function. Our previous studies have shown that electroacupuncture (EA) at the Jianshi–Neiguan acupoints (P5–P6, underlying the median nerve) inhibits central sympathetic outflow and attenuates excitatory cardiovascular reflexes, in part, through an opioid mechanism. It is unknown if EA at these acupoints influences the parasympathetic system. Thus, using c-Fos expression, we examined activation of nucleus ambiguus (NAmb) neurons by EA, their relation to cholinergic (preganglionic parasympathetic) neurons and those containing enkephalin. To enhance detection of cell bodies containing enkephalin, colchicine (90–100 μg/kg) was administered into the subarachnoid space of cats 30 hr prior to EA or sham-operated controls for EA. Following bilateral barodenervation and cervical vagotomy, either EA for 30 min at P5–P6 acupoints or control stimulation (needle placement at P5–P6 without stimulation) was applied. While perikarya containing enkephalin were observed in some medullary nuclei (e.g., râphe), only enkephalin-containing neuronal processes were found in the NAmb. Compared to controls (n=4), more c-Fos immunoreactivity, located principally in close proximity to fibers containing enkephalin was noted in the NAmb of EA-treated cats (n=5; P<0.01). Moreover, neurons double-labeled with c-Fos and choline acetyltransferase in the NAmb were identified in EA-treated, but not the control animals. These data demonstrate for the first time that EA activates preganglionic parasympathetic neurons in the NAmb. Because of their close proximity, these EA-activated neurons likely interact with nerve fibers containing enkephalin. These results suggest that EA at the P5–P6 acupoints has the potential to influence parasympathetic outflow and cardiovascular function, likely through an enkephalinergic mechanism.
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影响因子：
1.7
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