Association of Clubroot Resistance Locus PbBa8.1 With a Linkage Drag of High Erucic Acid Content in the Seed of the European Turnip
Association of Clubroot Resistance Locus PbBa8.1 With a Linkage Drag of High Erucic Acid Content in the Seed of the European Turnip
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欧洲萝卜种子中根肿病抗性位点PbBa8.1与高芥酸含量连锁拖动的关联
DOI：
10.3389/fpls.2020.00810
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发表时间：
2020-06
影响因子：
5.6
通讯作者：
Chunyu Zhang
中科院分区：
文献类型：
--
作者：
Zongxiang Zhan;Yingfen Jiang;Nadil Shah;Zhaoke Hou;Yuanwei Zhou;Bicheng Dun;Shisheng Li;Li Zhu;Zaiyun Li;Zhongyun Piao;Chunyu Zhang
根肿病是一种严重威胁油菜生产的病害。培育抗病品种是限制疾病传播的最有效和最环保的方法。我们研制出了一种高度耐药的B。甘蓝型油菜品系ZHE 226含有PbBa 8.1抗性位点。然而，ZHE 226种子含有较高的芥酸含量，这限制了其栽培，因为其食用油品质较低。以欧洲芜菁的抗芥酸供体ECD 04为母本，以种子中不含芥酸的优良品种黄双5号为轮回植株，构建了BC 3F 2分离群体。使用批量分离分析测序（BSA-Seq）方法的精细作图在染色体A08上的2.9 MB区域内检测到PbBa 8.1。有趣的是，先前报道的抗性基因Crr 1a也在同一区域被发现。遗传分析表明，Crr 1a的CAP-134标记与根肿病抗性（CR）紧密连锁。PbBa8.1和Crr 1a可能是CR的等位基因。比较分析和遗传分析表明，ZHE 226籽粒中高芥酸是由于ECD 04中脂肪酸延伸酶1（FAE 1）的连锁拖曳所致，位于PbBa 8.1区间，物理距离和遗传距离分别为729 Kb和1.86 cm。最后，通过基因特异分子标记辅助选择，从BC 4F 4中成功选育出了一个低芥酸抗根肿病品系。这些结果将加速B的CR育种计划。油菜。
Clubroot caused by Plasmodiophora brassicae is a severe threat to the production of Brassica napus, worldwide. The cultivation of resistant varieties is the most efficient and environmentally friendly way to limit disease spread. We developed a highly resistant B. napus line, ZHE226, containing the resistance locus PbBa8.1. However, ZHE226 seeds contain high erucic acid content, which limits its cultivation owing to its low edible oil quality. A segregation population of BC3F2 was developed by crossing ECD04, a resistant European turnip donor, with Huangshuang5, an elite variety with no erucic acid in its seeds, as a recurrent plant. Fine mapping using the bulk segregation analysis sequencing (BSA-Seq) approach detected PbBa8.1 within a 2.9 MB region on chromosome A08. Interestingly, the previously reported resistance gene Crr1a was found in the same region. Genetic analysis revealed that the CAP-134 marker for Crr1a was closely linked with clubroot resistance (CR). Thus, PbBa8.1 and Crr1a might be allelic for CR. Moreover, comparative and genetic analysis showed that high erucic acid in the seeds of ZHE226 was due to linkage drag of fatty acid elongase 1 (FAE1) in the ECD04 line, which was located in the interval of PbBa8.1 with a physical and genetic distance of 729 Kb and 1.86 cm, respectively. Finally, a clubroot-resistant line with a low erucic acid content was successfully developed through gene-specific molecular marker assistant selection from BC4F4. These results will accelerate CR breeding programs in B. napus.
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DOI：
10.1105/tpc.7.3.309
发表时间：
1995-03
期刊：
The Plant cell
影响因子：
--
作者：
D. W. James;E. Lim;J. Keller;Ingrid Plooy;E. Ralston;H. Dooner
通讯作者：
D. W. James;E. Lim;J. Keller;Ingrid Plooy;E. Ralston;H. Dooner
影响因子：
2.7
作者：
S. Hwang;H. Ahmed;Q. Zhou;S. Strelkov;B. Gossen;G. Peng;G. Turnbull
通讯作者：
S. Hwang;H. Ahmed;Q. Zhou;S. Strelkov;B. Gossen;G. Peng;G. Turnbull
长链单不饱和脂肪酸和2个前瞻性队列中充血性心力衰竭的发生率。
影响因子：
37.8
作者：
Imamura F;Lemaitre RN;King IB;Song X;Steffen LM;Folsom AR;Siscovick DS;Mozaffarian D
通讯作者：
Mozaffarian D
影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
DOI：
--
发表时间：
2009-08
期刊：
--
影响因子：
--
作者：
S. Crawford
通讯作者：
S. Crawford