Comparative Analysis of Nkx2-5/GATA4/TBX5 Expression in Chicken, Quail and Chicken-quail Hybrids during the Early Stage of Cardiac Development in Embryos.
Comparative Analysis of Nkx2-5/GATA4/TBX5 Expression in Chicken, Quail and Chicken-quail Hybrids during the Early Stage of Cardiac Development in Embryos.
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DOI：
10.5713/ajas.2012.12626
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Jia B
中科院分区：
文献类型：
--
作者：
Ban Q;Liu X;Hui W;Chen D;Zhao Z;Jia B
本研究对鸡、鹌鹑及鸡-鹌鹑杂种胚胎发育早期心脏发育相关基因的表达进行了研究。采用Real-time PCR方法检测鸡、鹌鹑和鸡-鹌鹑杂交胚胎在孵育第3 ~ 7天心脏Nkx 2 -5、GATA 4和TBX 5 mRNA的表达。结果表明，NKX 2 -5 mRNA在鸡、鹌鹑和鸡-鹌鹑杂种中的表达趋势相似。Nkx 2 -5的表达量在第3天达到最高，第5天开始下降，此后出现波动。Nkx 2 -5基因在鹌鹑中的表达量显著高于鸡和鸡-鹌鹑杂交种，而在鸡-鹌鹑杂交种中的表达量无显著差异。GATA 4 mRNA在鸡和鹌鹑中的表达趋势相似，在第3 ~ 6 d呈现稳定的上升趋势，随后表达水平下降。鸡-鹌鹑杂交后代GATA 4 mRNA表达量在3 ~ 5 d显著高于鸡和鹌鹑（p<0.01），但在试验后期显著低于鸡和鹌鹑（p<0.05），从5 d开始显著下降（p<0.01）。TBX 5 mRNA在鸡和鹌鹑中的表达趋势与GATA 4相同。在整个试验过程中，鸡-鹌鹑杂交后代TBX 5的表达量显著高于鸡和鹌鹑，而在试验早期TBX 5的表达量相对较低。因此，Nkx 2 -5、GATA 4和TBX 5基因在鸡、鹌鹑及其杂种胚胎心脏发育过程中呈现动态变化。此外，在鸡中的表达趋势与鹌鹑相似，除NKX 2 -5外，其他基因的表达水平均无显著差异。但这些基因在鸡-鹌鹑杂交后代中的表达与其亲本存在显著差异，其差异机制有待进一步探讨。
The present study makes an investigation into expression of genes related to cardiac development in chicken, quail and chicken-quail hybrids during the early stage of embryogenesis. Real-time PCR was used to detect mRNA expressions of Nkx2-5, GATA4 and TBX5 in the heart of chicken, quail and chicken-quail hybrids embryos during the 3rd to 7th days of incubation. Results showed that NKX2-5 mRNA displayed a similar expression trend in chicken, quail and chicken-quail hybrids. The initial and highest expression of Nkx2-5 was focused on the 3rd day of incubation, then it declined till 5th day of incubation, thereafter, it fluctuated. Expression of Nkx2-5 gene in quail was significantly higher than in chicken and chicken-quail hybrids, and no significant difference was observed between the two latter species. GATA4 mRNA showed a similar expression trend between chicken and quail, which displayed a steady increase from 3rd to 6th d, then, the expression level decreased. However, GATA4 mRNA expression in chicken-quail hybrids was significantly higher than that in chicken and quail from 3rd to 5th d (p<0.01), but significantly lower than that in chicken and quail during the later stage of the experiment (p<0.05), due to the dramatic drop from 5th d onwards (p<0.01). TBX5 mRNA expression in chicken and quail showed the same trend as GATA4 expressed in the two species. Furthermore, TBX5 expression in chicken-quail hybrids was significantly higher than that in chicken and quail during the whole course of experiment, although relatively lower TBX5 expression was detected in the early stage. In conclusion, Nkx2-5, GATA4 and TBX5 genes showed dynamic changes during the process of cardiac development in chicken, quail and their hybrids embryos. In addition, the expression trend in chicken was similar to that in quail, and there was no significant difference for gene expression level, except NKX2-5. However, expression of these genes in chicken-quail hybrids was significantly different from their parents, the difference mechanism needs to be further explored.
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影响因子：
2.6
作者：
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DOI：
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