Phylogeny and Toxin Profile of Freshwater Pufferfish (Genus Pao) Collected from 2 Different Regions in Cambodia.
Phylogeny and Toxin Profile of Freshwater Pufferfish (Genus Pao) Collected from 2 Different Regions in Cambodia.
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DOI：
10.3390/toxins12110689
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发表时间：
2020-10-30
期刊：
影响因子：
4.2
通讯作者：
Arakawa O
中科院分区：
文献类型：
--
作者：
Zhu H;Yamada A;Goto Y;Horn L;Ngy L;Wada M;Doi H;Lee JS;Takatani T;Arakawa O
柬埔寨淡水河豚的种类分类是不完整和混乱的，关于它们的毒性和毒素的科学信息是有限的。在本研究中，为了积累有关淡水河豚的系统发育和毒素特征的信息，并为柬埔寨的食品安全做出贡献，我们同时对从金边和Kratie（分别指定为PNH和KTI）收集的淡水河豚进行了基于遗传学的系统发育和毒素分析。对3个线粒体基因（细胞色素b、16S rRNA和细胞色素c氧化酶亚基I）的部分序列进行系统发育分析，发现PNH和KTI属于Pao属（分别命名为Pao sp. A和Pao sp. b）的不同种。核三丁基锡结合蛋白2 （TBT-bp2）基因的部分序列在氨基酸水平上区分了物种。毒素谱分析表明，Pao sp. A和Pao sp. B均含有以STX为主要成分的saxitoxins （STXs）。在Pao sp. A中，每个组织中的毒素浓度非常高，远远超过法典委员会规定的stx的监管限值，而在Pao sp. B中，只有皮肤含有高浓度毒素。不同TBT-bp2基因序列的两种河豚体内STX积累能力的差异表明TBT-bp2基因参与了淡水河豚体内STX的积累。
The species classification of Cambodian freshwater pufferfish is incomplete and confusing, and scientific information on their toxicity and toxin profile is limited. In the present study, to accumulate information on the phylogeny and toxin profile of freshwater pufferfish, and to contribute to food safety in Cambodia, we conducted simultaneous genetic-based phylogenetic and toxin analyses using freshwater pufferfish individuals collected from Phnom Penh and Kratie (designated PNH and KTI, respectively). Phylogenetic analysis of partial sequences of three mitochondrial genes (cytochrome b, 16S rRNA, and cytochrome c oxidase subunit I) determined for each fish revealed that PNH and KTI are different species in the genus Pao (designated Pao sp. A and Pao sp. B, respectively). A partial sequence of the nuclear tributyltin-binding protein type 2 (TBT-bp2) gene differentiated the species at the amino acid level. Instrumental analysis of the toxin profile revealed that both Pao sp. A and Pao sp. B possess saxitoxins (STXs), comprising STX as the main component. In Pao sp. A, the toxin concentration in each tissue was extremely high, far exceeding the regulatory limit for STXs set by the Codex Committee, whereas in Pao sp. B, only the skin contained high toxin concentrations. The difference in the STX accumulation ability between the two species with different TBT-bp2 sequences suggests that TBT-bp2 is involved in STX accumulation in freshwater pufferfish.
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DOI：
10.3358/shokueishi.40.5_363
发表时间：
1999-10-01
期刊：
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
影响因子：
--
作者：
Mahmud, Y;Yamamori, K;Noguchi, T
通讯作者：
Noguchi, T
影响因子：
3.2
作者：
Barrientos, Rocio Gonzalez;Hernandez-Mora, Gabriela;Stacy, Brian A.
通讯作者：
Stacy, Brian A.
影响因子：
2.8
作者：
Nakashima, K;Arakawa, O;Noguchi, T
通讯作者：
Noguchi, T
影响因子：
2.7
作者：
Oba, Yumi;Shimasaki, Yohei;Honjo, Tsuneo
通讯作者：
Honjo, Tsuneo
影响因子：
2.8
作者：
Etheridge, Stacey M.
通讯作者：
Etheridge, Stacey M.