Efficiency of vanilla, patchouli and ylang ylang essential oils stabilized by iron oxide@C14 nanostructures against bacterial adherence and biofilms formed by Staphylococcus aureus and Klebsiella pneumoniae clinical strains.
Efficiency of vanilla, patchouli and ylang ylang essential oils stabilized by iron oxide@C14 nanostructures against bacterial adherence and biofilms formed by Staphylococcus aureus and Klebsiella pneumoniae clinical strains.
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DOI：
10.3390/molecules191117943
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发表时间：
2014-11-04
期刊：
影响因子：
--
通讯作者：
Chifiriuc MC
中科院分区：
文献类型：
--
作者：
Bilcu M;Grumezescu AM;Oprea AE;Popescu RC;Mogoșanu GD;Hristu R;Stanciu GA;Mihailescu DF;Lazar V;Bezirtzoglou E;Chifiriuc MC
细菌细胞形成的生物膜与对大多数抗菌剂的耐药性显著增强有关，导致微生物感染的持久性和慢性化，并导致治疗失败。本研究的目的是将磁性纳米粒子的独特性质与三种精油的抗菌活性相结合，获得新型纳米生物系统，用于导管件涂层，提高对金黄色葡萄球菌和肺炎克雷伯菌的抵抗力，促进临床菌株的黏附和生物被膜的形成。伊兰精油、广霍香精油和香草精油通过与纳米氧化铁@C14的相互作用而稳定下来，进一步用作医用表面的涂层剂。在碱性介质中，通过Fe+2和Fe+3与肉豆蔻酸(C14)的共沉淀制备了氧化铁@C14。胶囊化的香草精油纳米粒对金黄色葡萄球菌细胞的初始黏附和48h金黄色葡萄球菌成熟生物膜的形成均有很强的抑制作用，广霍香和依兰精油主要抑制金黄色葡萄球菌生物膜形成的初始黏附阶段。在肺炎克雷伯菌的案例中，所有测试的纳米系统都显示出类似的效率，主要是对肺炎克雷伯菌细胞与测试导管样本的粘连起到了抑制作用。基于香草精油、广霍香精油和依兰精油的新型纳米生物体系可能会引起生物医学领域的极大兴趣，为设计具有防粘连和抗生物被膜性能的薄膜涂层表面开辟了新的方向。
Biofilms formed by bacterial cells are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence and chronicization of the microbial infections and to therapy failure. The purpose of this study was to combine the unique properties of magnetic nanoparticles with the antimicrobial activity of three essential oils to obtain novel nanobiosystems that could be used as coatings for catheter pieces with an improved resistance to Staphylococcus aureus and Klebsiella pneumoniae clinical strains adherence and biofilm development. The essential oils of ylang ylang, patchouli and vanilla were stabilized by the interaction with iron oxide@C14 nanoparticles to be further used as coating agents for medical surfaces. Iron oxide@C14 was prepared by co-precipitation of Fe+2 and Fe+3 and myristic acid (C14) in basic medium. Vanilla essential oil loaded nanoparticles pelliculised on the catheter samples surface strongly inhibited both the initial adherence of S. aureus cells (quantified at 24 h) and the development of the mature biofilm quantified at 48 h. Patchouli and ylang-ylang essential oils inhibited mostly the initial adherence phase of S. aureus biofilm development. In the case of K. pneumoniae, all tested nanosystems exhibited similar efficiency, being active mostly against the adherence K. pneumoniae cells to the tested catheter specimens. The new nanobiosystems based on vanilla, patchouli and ylang-ylang essential oils could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with anti-adherence and anti-biofilm properties.
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影响因子：
2.3
作者：
Chifiriuc, Mariana Carmen;Grumezescu, Alexandru Mihai;Radulescu, Radu
通讯作者：
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影响因子：
3.9
作者：
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影响因子：
--
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通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
5.6
作者：
Anghel I;Grumezescu AM;Holban AM;Ficai A;Anghel AG;Chifiriuc MC
通讯作者：
Chifiriuc MC