The involvement of auxin in root architecture plasticity in Arabidopsis induced by heterogeneous phosphorus availability
The involvement of auxin in root architecture plasticity in Arabidopsis induced by heterogeneous phosphorus availability
复制标题
生长素参与拟南芥异质磷有效性诱导的根结构可塑性
DOI：
10.1007/s10535-013-0327-z
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发表时间：
2013-04
影响因子：
1.5
通讯作者：
Jinxing Wang
中科院分区：
文献类型：
--
作者：
Qian Liu;Guoquan Zhou;Xu Feng;Xiaolong Yan;Hong Liao;Jinxing Wang
研究表明，低磷可通过生长素调控拟南芥根构型，但生长素在根构型对磷异质性的可塑性中的作用尚不清楚。在这项研究中，我们采用生长素的生物合成，运输和信号相关的突变体。首先，我们发现，与低磷（LP）含量的全培养基相比，将拟南芥的初生根（PR）分为上、下两部分，即上、下两部分，高磷（HP）含量或相反，可以部分地挽救其生长。下部LP比上部LP更有效地阻止PR生长以及降低侧根密度（DLR），并且效果依赖于生长素极性运输。其次，我们证实了生长素受体TIR1参与了PR生长和侧根发育对磷供应的反应，TIR1功能丧失抑制侧根发育。第三，异质磷对PR上部LRD的影响依赖于PIN2和PIN4，对PR下部LRD的影响依赖于PIN3和PIN4，而对总LRs密度的影响依赖于生长素转运蛋白PIN2和PIN7。最后，异质磷供应改变了PR尖生长素的积累和生长素生物合成相关基因TAA1，YUC 1，YUC 2和YUC 4的表达。综上所述，我们提供的证据表明，生长素参与根构型可塑性响应异质磷供应在拟南芥。
Homogeneous low phosphorus availability was reported to regulate root architecture in Arabidopsis via auxin, but the roles of auxin in root architecture plasticity to heterogeneous P availability remain unclear. In this study, we employed auxin biosynthesis-, transport- and signalling-related mutants. Firstly, we found that in contrast to low P (LP) content in the whole medium, primary root (PR) growth of Arabidopsis was partially rescued in the medium divided into two parts: upper with LP and lower with high P (HP) content or in the reverse arrangement. The down part LP was more effective to arrest PR growth as well as to decrease density of lateral roots (DLR) than the upper LP, and effects were dependent on polar auxin transport. Secondly, we verified that auxin receptor TIR1 was involved in the responses of PR growth and lateral root (LR) development to P supply and loss of function of TIR1 inhibited LR development. Thirdly, effects of heterogeneous P on LRD in the upper part of PR was dependent on PIN2 and PIN4, and in the down part on PIN3 and PIN4, whereas density of total LRs was dependent on auxin transporters PIN2 and PIN7. Finally, heterogeneous P availability altered the accumulation of auxin in PR tip and the expression of auxin biosynthesisrelated genes TAA1, YUC1, YUC2, and YUC4. Taken together, we provided evidences for the involvement of auxin in root architecture plasticity in response to heterogeneous phosphorus availability in Arabidopsis.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
5.3
作者：
Woo J;MacPherson CR;Liu J;Wang H;Kiba T;Hannah MA;Wang XJ;Bajic VB;Chua NH
通讯作者：
Chua NH
影响因子：
11.6
作者：
FERREIRA, PCG;HEMERLY, AS;INZE, D
通讯作者：
INZE, D
影响因子：
11.6
作者：
Casimiro, I;Marchant, A;Bennett, M
通讯作者：
Bennett, M
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde