Culture and co-culture of mouse ovaries and ovarian follicles.
Culture and co-culture of mouse ovaries and ovarian follicles.
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DOI：
10.3791/52458
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发表时间：
2015-03-17
期刊：
影响因子：
--
通讯作者：
Spears N
中科院分区：
文献类型：
--
作者：
Morgan S;Campbell L;Allison V;Murray A;Spears N
哺乳动物的卵巢由卵泡组成，每个卵泡由单个卵母细胞组成，卵母细胞被体细胞颗粒细胞包围，体细胞颗粒细胞一起封闭在基底膜内。在胚胎发育过程中，当减数分裂停滞的卵母细胞与扁平颗粒细胞形成紧密结合时，形成有限的卵泡池，形成原始卵泡。哺乳动物出生时或出生后不久，卵巢中含有它们一生所需的原始卵泡，从这一点开始，卵泡稳定地释放到不断生长的卵泡池中。卵巢特别适合于体外发育，卵泡在培养中以高度生理方式生长。这项工作描述了培养含有原始卵泡的整个新生儿卵巢，以及培养单个卵泡，这种方法可以支持卵泡从不成熟到排卵前阶段的发育，之后它们的卵母细胞能够在体外受精。这里概述的工作使用培养系统来确定卵巢如何受到外部化合物的影响。我们还描述了一个共培养系统，它允许调查之间发生的相互作用，生长卵泡和非生长池的原始卵泡。
The mammalian ovary is composed of ovarian follicles, each follicle consisting of a single oocyte surrounded by somatic granulosa cells, enclosed together within a basement membrane. A finite pool of follicles is laid down during embryonic development, when oocytes in meiotic arrest form a close association with flattened granulosa cells, forming primordial follicles. By or shortly after birth, mammalian ovaries contain their lifetime’s supply of primordial follicles, from which point onwards there is a steady release of follicles into the growing follicular pool. The ovary is particularly amenable to development in vitro, with follicles growing in a highly physiological manner in culture. This work describes the culture of whole neonatal ovaries containing primordial follicles, and the culture of individual ovarian follicles, a method which can support the development of follicles from an immature through to the preovulatory stage, after which their oocytes are able to undergo fertilization in vitro. The work outlined here uses culture systems to determine how the ovary is affected by exposure to external compounds. We also describe a co-culture system, which allows investigation of the interactions that occur between growing follicles and the non-growing pool of primordial follicles.
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影响因子：
3.6
作者：
Campbell, Lisa;Trendell, Jenny;Spears, Norah
通讯作者：
Spears, Norah
DOI：
10.1530/jrf.0.1060001
发表时间：
1996-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Spears, N;deBruin, JP;Gosden, RG
通讯作者：
Gosden, RG
影响因子：
3.6
作者：
O'Brien, MJ;Pendola, JK;Eppig, JJ
通讯作者：
Eppig, JJ
影响因子：
16.2
作者：
Feng, GP;Mellor, RH;Sanes, JR
通讯作者：
Sanes, JR
影响因子：
2.7
作者：
Pratt, T;Sharp, L;Mason, JO
通讯作者：
Mason, JO