Different migration patterns of sea urchin and mouse sperm revealed by a microfluidic chemotaxis device.
Different migration patterns of sea urchin and mouse sperm revealed by a microfluidic chemotaxis device.
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DOI：
10.1371/journal.pone.0060587
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wu M
中科院分区：
文献类型：
--
作者：
Chang H;Kim BJ;Kim YS;Suarez SS;Wu M
趋化性是指细胞沿着化学梯度向上或向下移动的过程。精子趋化性是海洋无脊椎动物（如海胆）利用的一种策略，可以在流动的水中有效地到达卵子。哺乳动物精子如何或是否利用趋化性到达卵子，受精发生在生殖道的限制内，对此了解较少。在本报告中，我们使用最近开发的微流体模型和高速成像定量评估了海胆和小鼠精子的趋化性。结果表明，海胆Arbacia punctulata精子具有高趋化敏感性，其在化学梯度上的平均速度Vx高达其平均速度（238 μm/s）的20%;而小鼠精子在孕酮梯度上的趋化行为无统计学意义。这项工作证明了定量精子趋化性研究的微流体模型的有效性，并揭示了一个生物学的见解，趋化性的孕酮梯度可能不是一个通用的策略，哺乳动物精子到达卵子。
Chemotaxis refers to a process whereby cells move up or down a chemical gradient. Sperm chemotaxis is known to be a strategy exploited by marine invertebrates such as sea urchins to reach eggs efficiently in moving water. Less is understood about how or whether chemotaxis is used by mammalian sperm to reach eggs, where fertilization takes place within the confinement of a reproductive tract. In this report, we quantitatively assessed sea urchin and mouse sperm chemotaxis using a recently developed microfluidic model and high-speed imaging. Results demonstrated that sea urchin Arbacia punctulata sperm were chemotactic toward the peptide resact with high chemotactic sensitivity, with an average velocity Vx up the chemical gradient as high as 20% of its average speed (238 μm/s), while mouse sperm displayed no statistically significant chemotactic behavior in progesterone gradients, which had been proposed to guide mammalian sperm toward eggs. This work demonstrates the validity of a microfluidic model for quantitative sperm chemotaxis studies, and reveals a biological insight that chemotaxis up a progesterone gradient may not be a universal strategy for mammalian sperm to reach eggs.
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