Biomimetic extracellular matrix coatings improve the chronic biocompatibility of microfabricated subdural microelectrode arrays.
Biomimetic extracellular matrix coatings improve the chronic biocompatibility of microfabricated subdural microelectrode arrays.
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DOI：
10.1371/journal.pone.0206137
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Litt B
中科院分区：
文献类型：
--
作者：
Vitale F;Shen W;Driscoll N;Burrell JC;Richardson AG;Adewole O;Murphy B;Ananthakrishnan A;Oh H;Wang T;Lucas TH;Cullen DK;Allen MG;Litt B
颅内电极是植入式神经设备的重要组成部分，无论是用于急性诊断还是用于开环和闭环神经调节的慢性治疗。它们的性能受到急性植入创伤和对植入材料的慢性炎症的影响，以及僵硬的合成电极和脉动的自然软宿主神经组织之间的机械不匹配。基于具有微尺度导电特征的聚合物薄膜的柔性电子器件可以帮助减轻机械诱导的创伤；然而，仅凭这一策略并不能减轻设备-组织界面的炎症。在这项研究中，我们提出了一种仿生方法，将微尺度的细胞外基质(ECM)涂层集成到微制造的柔性硬膜下微电极上。利用采用微转移模塑和准分子激光微加工的高通量工艺，我们制备了主要由ECM蛋白质材料组成的多通道硬膜下微电极，并证明了其电化学和机械性能与标准的、未涂覆的对照相匹配。在啮齿动物大脑中的活体ECoG记录证实，ECM微电极涂层和蛋白质界面不会改变信号保真度。在大鼠体感皮质硬膜下植入7天和30天后，与未涂膜的对照组相比，对ECM涂层装置的星形胶质异物反应减少。我们提出了微细加工、柔性、仿生电极作为一种新的策略，以减少装置-组织界面的炎症，并提高可植入硬膜下电极的长期稳定性。
Intracranial electrodes are a vital component of implantable neurodevices, both for acute diagnostics and chronic treatment with open and closed-loop neuromodulation. Their performance is hampered by acute implantation trauma and chronic inflammation in response to implanted materials and mechanical mismatch between stiff synthetic electrodes and pulsating, natural soft host neural tissue. Flexible electronics based on thin polymer films patterned with microscale conductive features can help alleviate the mechanically induced trauma; however, this strategy alone does not mitigate inflammation at the device-tissue interface. In this study, we propose a biomimetic approach that integrates microscale extracellular matrix (ECM) coatings on microfabricated flexible subdural microelectrodes. Taking advantage of a high-throughput process employing micro-transfer molding and excimer laser micromachining, we fabricate multi-channel subdural microelectrodes primarily composed of ECM protein material and demonstrate that the electrochemical and mechanical properties match those of standard, uncoated controls. In vivo ECoG recordings in rodent brain confirm that the ECM microelectrode coatings and the protein interface do not alter signal fidelity. Astrogliotic, foreign body reaction to ECM coated devices is reduced, compared to uncoated controls, at 7 and 30 days, after subdural implantation in rat somatosensory cortex. We propose microfabricated, flexible, biomimetic electrodes as a new strategy to reduce inflammation at the device-tissue interface and improve the long-term stability of implantable subdural electrodes.
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影响因子：
17.1
作者：
Jarosiewicz B;Sarma AA;Bacher D;Masse NY;Simeral JD;Sorice B;Oakley EM;Blabe C;Pandarinath C;Gilja V;Cash SS;Eskandar EN;Friehs G;Henderson JM;Shenoy KV;Donoghue JP;Hochberg LR
通讯作者：
Hochberg LR
影响因子：
4.6
作者：
Franks, W;Schenker, I;Hierlemann, A
通讯作者：
Hierlemann, A
影响因子：
4.6
作者：
Bouafsoun, Amira;Helali, Saloua;Ponsonnet, Laurence
通讯作者：
Ponsonnet, Laurence
影响因子：
25
作者：
Khodagholy, Dion;Gelinas, Jennifer N.;Thesen, Thomas;Doyle, Werner;Devinsky, Orrin;Malliaras, George G.;Buzsaki, Gyoergy
通讯作者：
Buzsaki, Gyoergy
DOI：
10.3389/fneng.2014.00007
发表时间：
2014-01-01
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
De Faveri, Sara;Maggiolini, Emma;Fadiga, Luciano
通讯作者：
Fadiga, Luciano