Protective immunity against Schistosoma japonicum infection can be provided by IgG antibodies towards periodate-sensitive or periodate-resistant glycans.
Protective immunity against Schistosoma japonicum infection can be provided by IgG antibodies towards periodate-sensitive or periodate-resistant glycans.
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针对高碘酸盐敏感或高碘酸盐抗性聚糖的 IgG 抗体可以提供针对日本血吸虫感染的保护性免疫。
DOI：
10.1186/s13071-015-0842-1
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发表时间：
2015-04-18
影响因子：
3.2
通讯作者：
Chen XP
中科院分区：
文献类型：
--
作者：
Gong W;Huang F;Ma Y;Bai H;Yin L;Li J;Chen C;Xu X;Chen XP
已被广泛接受的是，存在于核糖体中的聚糖是高度抗原性的。然而，目前尚不清楚什么样的蠕虫聚糖可以影响感染宿主的抗体IgG应答，以及抗体IgG应答是否具有保护性。通过在来自感染小鼠和人的血清中使用竞争性ELISA测定来测量聚糖的抗原性贡献。用日本血吸虫虫卵可溶性抗原（SjEA）免疫小鼠，制备了抗SjEA的单克隆抗体。用免疫荧光法检测尾蚴和幼虫表面多糖的表达和分布。通过测定感染小鼠的蠕虫和虫卵负荷来测定聚糖特异性mAb的保护作用。高碘酸盐抗性聚糖和高碘酸盐敏感性聚糖都是嗜酸性粒细胞感染中的抗原性。当在小鼠中的噬菌体感染之前施用针对高碘酸盐敏感性或高碘酸盐抗性聚糖的单克隆抗体时，发现两种抗聚糖抗体都成功地为感染的小鼠提供保护性免疫。高碘酸盐抗性和高碘酸盐敏感性聚糖都是抗原性的，并且显性抗聚糖IgG应答可以在受高碘酸盐感染的宿主中的保护性免疫中起重要作用。本文的在线版本（doi：10.1186/s13071-015-0842-1）包含补充材料，可供授权用户使用。
It has been well accepted that glycans present in schistosomes are highly antigenic. However, it is not clear what kind of worm glycans can affect the infected host to mount IgG responses and whether mounted anti-glycan IgG responses are protective. The contribution of antigenicity by glycans was measured by using competitive ELISA assay in sera from infected mice and humans. Monoclonal antibodies towards soluble Schistosoma japonicum egg antigens (SjEA) were generated from SjEA immunizated mice. The expression of glycans on surfaces of cercaria or young worm and their distributions were examined by immunofluorescence assay. The protective roles of glycans-specific mAbs were assayed by determination of the worm and egg burden in infected mice. Both periodate-resistant glycans and periodate-sensitive glycans are antigenic in schistosome infections. When monoclonal antibodies against either periodate-sensitive or periodate-resistant glycans were administered prior to schistosome infections in mice, both kinds of anti-glycan antibodies were found to successfully provide protective immunity to infected mice. Both periodate-resistant and periodate-sensitive glycans are antigenic, and dominant anti-glycan IgG responses can play important roles in protective immunity in schistosome infected hosts. The online version of this article (doi:10.1186/s13071-015-0842-1) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
van Diepen A;Smit CH;van Egmond L;Kabatereine NB;Pinot de Moira A;Dunne DW;Hokke CH
通讯作者：
Hokke CH
DOI：
10.1084/jem.165.3.865
发表时间：
1987-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Grzych JM;Dissous C;Capron M;Torres S;Lambert PH;Capron A
通讯作者：
Capron A
影响因子：
4.3
作者：
Nyame, AK;Leppanen, AM;Cummings, RD
通讯作者：
Cummings, RD
影响因子：
2.2
作者：
BICKLE, QD;FORD, MJ;ANDREWS, BJ
通讯作者：
ANDREWS, BJ
影响因子：
4.3
作者：
Nyame, AK;Debose-Boyd, R;Cummings, RD
通讯作者：
Cummings, RD