Obesity occurring in apolipoprotein E-knockout mice has mild effects on fertility.
Obesity occurring in apolipoprotein E-knockout mice has mild effects on fertility.
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载脂蛋白 E 敲除小鼠发生的肥胖对生育能力有轻微影响。
DOI：
10.1530/rep-13-0470
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发表时间：
2013-12
期刊：
影响因子：
3.8
通讯作者：
Zhang, Cong
中科院分区：
文献类型：
--
作者：
Liu, Jianwei;Pan, Jie;Yang, Guiwen;Zhang, Cong
载脂蛋白(Apo)家族与脂质代谢有关。载脂蛋白有五种类型：载脂蛋白A、载脂蛋白B、载脂蛋白C、载脂蛋白D和载脂蛋白E。载脂蛋白E已被证明在脂蛋白代谢中发挥中心作用，并对受体介导的血浆有效清除肝脏中乳糜粒残留物和极低密度脂蛋白残留物至关重要。APOE缺陷(APOE(-/-))小鼠自发形成动脉粥样硬化斑块，随后发生肥胖。在这项研究中，我们调查了APOE基因敲除引起的脂肪沉积是否影响雌性小鼠的生殖。结果表明，APOE(-/-)小鼠存在严重的高胆固醇血症，胆固醇代谢紊乱，卵巢内脂肪堆积，导致卵巢比WT小鼠更重。此外，雌激素和孕酮在第100天显著下降。定量聚合酶链式反应分析显示，在D100时，APOE(-/-)小鼠细胞色素P450芳香化酶、3-β-羟基类固醇脱氢酶、雷帕霉素机制靶点和核因子-κB的表达显著降低，而bcl2相关的细胞死亡激动剂和结节性硬化症复合体2的表达显著增加。然而，APOE(-/-)和WT小鼠的生育率没有差异；也就是说，APOE基因敲除导致的肥胖对生殖没有显著影响。但是，APOE基因的缺失增加了卵泡的数量，增加了卵泡闭锁和凋亡的比例。我们相信，这项工作将增进我们对载脂蛋白E在生殖中的作用的理解。
The Apolipoprotein (Apo) family is implicated in lipid metabolism. There are five types of Apo: Apoa, Apob, Apoc, Apod, and Apoe. Apoe has been demonstrated to play a central role in lipoprotein metabolism and to be essential for efficient receptor-mediated plasma clearance of chylomicron remnants and VLDL remnant particles by the liver. Apoe-deficient (Apoe(-/-)) mice develop atherosclerotic plaques spontaneously, followed by obesity. In this study, we investigated whether lipid deposition caused by Apoe knockout affects reproduction in female mice. The results demonstrated that Apoe(-/-) mice were severely hypercholesterolemic, with their cholesterol metabolism disordered, and lipid accumulating in the ovaries causing the ovaries to be heavier compared with the WT counterparts. In addition, estrogen and progesterone decreased significantly at D 100. Quantitative PCR analysis demonstrated that at D 100 the expression of cytochromeP450 aromatase (Cyp19a1), 3β-hydroxysteroid dehydrogenase (Hsd3b), mechanistic target of rapamycin (Mtor), and nuclear factor-κB (Nfkb) decreased significantly, while that of BCL2-associated agonist of cell death (Bad) and tuberous sclerosis complex 2 (Tsc2) increased significantly in the Apoe(-/-) mice. However, there was no difference in the fertility rates of the Apoe(-/-) and WT mice; that is, obesity induced by Apoe knockout has no significant effect on reproduction. However, the deletion of Apoe increased the number of ovarian follicles and the ratio of ovarian follicle atresia and apoptosis. We believe that this work will augment our understanding of the role of Apoe in reproduction.
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DOI：
10.1530/rep-09-0005
发表时间：
2010-01
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Bogan RL;Hennebold JD
通讯作者：
Hennebold JD
影响因子：
56.9
作者：
MAHLEY, RW
通讯作者：
MAHLEY, RW
影响因子：
10.5
作者：
YUE, L;SPRADLING, AC
通讯作者：
SPRADLING, AC
影响因子：
5.3
作者：
Gerdes, LU;Gerdes, C;Dyerberg, J
通讯作者：
Dyerberg, J
影响因子：
6.5
作者：
D. France;T. Hughes;R. Miserendino;J. A. Spirito;J. Babiak;J. B. Eskesen;C. Tapparelli;J. Paterniti
通讯作者：
D. France;T. Hughes;R. Miserendino;J. A. Spirito;J. Babiak;J. B. Eskesen;C. Tapparelli;J. Paterniti