Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon.
Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon.
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DOI：
10.1038/s41598-018-31107-z
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发表时间：
2018-08-23
影响因子：
4.6
通讯作者：
Rodríguez A
中科院分区：
文献类型：
--
作者：
Fernández L;González S;Quiles-Puchalt N;Gutiérrez D;Penadés JR;García P;Rodríguez A
金黄色葡萄球菌是一种主要的机会致病菌，通常在各种生物和非生物表面上形成生物膜。此外，已知大多数分离株在其基因组中携带前噬菌体。考虑到这一点，似乎更好地了解预言对S。金黄色葡萄球菌生物膜细胞将有助于制定消除这种病原体的策略。在这里，我们对由S. aureus RN 450和两株携带原噬菌体RN 411和RN 480 α的衍生菌株。溶源性菌株显示增加的生物膜形成和类胡萝卜素色素葡萄黄素的生产。这些表型可以部分解释由原噬菌体窝藏菌株，即交替西格玛因子（SigB）调节子的激活和Agr群体感应系统控制的基因的下调，特别是减少转录的基因编码的分散因子，如蛋白酶显示的基因表达的差异。尽管如此，部分种群的自发裂解也可能有助于增加附着的生物量。有趣的是，噬菌体Cl蛋白似乎在协调这些噬菌体-宿主相互作用中发挥作用，尽管需要更多的研究来证实这种可能性。同样，未来的研究应该检查这两个前噬菌体在感染过程中的影响。
Staphylococcus aureus is a major opportunistic pathogen that commonly forms biofilms on various biotic and abiotic surfaces. Also, most isolates are known to carry prophages in their genomes. With this in mind, it seems that acquiring a better knowledge of the impact of prophages on the physiology of S. aureus biofilm cells would be useful for developing strategies to eliminate this pathogen. Here, we performed RNA-seq analysis of biofilm cells formed by S. aureus RN450 and two derived strains carrying prophages ϕ11 and ϕ80α. The lysogenic strains displayed increased biofilm formation and production of the carotenoid pigment staphyloxanthin. These phenotypes could be partly explained by the differences in gene expression displayed by prophage-harboring strains, namely an activation of the alternative sigma factor (SigB) regulon and downregulation of genes controlled by the Agr quorum-sensing system, especially the decreased transcription of genes encoding dispersion factors like proteases. Nonetheless, spontaneous lysis of part of the population could also contribute to the increased attached biomass. Interestingly, it appears that the phage CI protein plays a role in orchestrating these phage-host interactions, although more research is needed to confirm this possibility. Likewise, future studies should examine the impact of these two prophages during the infection.
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影响因子：
4.6
作者：
Fernández L;González S;Campelo AB;Martínez B;Rodríguez A;García P
通讯作者：
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通讯作者：
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作者：
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通讯作者：
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作者：
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