Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level.
Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level.
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DOI：
10.1038/srep35646
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发表时间：
2016-10-19
影响因子：
4.6
通讯作者：
van der Woude MW
中科院分区：
文献类型：
--
作者：
Privat-Maldonado A;O'Connell D;Welch E;Vann R;van der Woude MW
低温等离子体（LTP）产生具有杀菌活性的活性氮和氧物质（RNOS）的混合物。然而，RNOS在空间上不均匀地分布在等离子体中。在这里，我们测试的假设，这种分布将影响机制的基础上的血浆杀菌活性集中在原位DNA损伤的水平。第一次，一个定量的，单细胞的方法被应用于评估细菌中的DNA损伤的水平作为从等离子体射流中心的径向距离的函数。固体，干燥表面上的肠道沙门氏菌进行了处理与两种类型的LTP：大气压介质阻挡放电等离子体射流（带电和中性物种）和射频大气压等离子体射流（中性物种）。在这两种情况下，DNA损伤的程度与等离子体中心的径向距离之间存在负相关性，最高的DNA损伤直接发生在等离子体下方。金黄色葡萄球菌也观察到这种趋势。LTP产生的紫外线辐射作为一个影响因素被排除。因此，可以从生物材料的测定中获得有价值的机制信息，这可以为LTP作为（局部）细菌感染的补充或替代疗法的开发提供信息。
Low temperature plasmas (LTPs) generate a cocktail of reactive nitrogen and oxygen species (RNOS) with bactericidal activity. The RNOS however are spatially unevenly distributed in the plasma. Here we test the hypothesis that this distribution will affect the mechanisms underpinning plasma bactericidal activity focussing on the level of DNA damage in situ. For the first time, a quantitative, single cell approach was applied to assess the level of DNA damage in bacteria as a function of the radial distance from the centre of the plasma jet. Salmonella enterica on a solid, dry surface was treated with two types of LTP: an atmospheric-pressure dielectric barrier discharge plasma jet (charged and neutral species) and a radio-frequency atmospheric-pressure plasma jet (neutral species). In both cases, there was an inverse correlation between the degree of DNA damage and the radial distance from the centre of the plasma, with the highest DNA damage occurring directly under the plasma. This trend was also observed with Staphylococcus aureus. LTP-generated UV radiation was eliminated as a contributing factor. Thus valuable mechanistic information can be obtained from assays on biological material, which can inform the development of LTP as a complementary or alternative therapy for (topical) bacterial infections.
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影响因子：
3.3
作者：
Leduc, M.;Guay, D.;Coulombe, S.
通讯作者：
Coulombe, S.
DOI：
10.1016/j.mrfmmm.2003.07.002
发表时间：
2003-10-29
影响因子：
2.3
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影响因子：
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DOI：
10.1016/j.ijantimicag.2013.08.022
发表时间：
2014-02-01
影响因子：
10.8
作者：
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通讯作者：
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影响因子：
3.3
作者：
Ellerweg, D.;Benedikt, J.;Schulz-von der Gathen, V.
通讯作者：
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