Simultaneous determination of two Monascus metabolites in red yeast rice by HPLC using fluorescence detection
Simultaneous determination of two Monascus metabolites in red yeast rice by HPLC using fluorescence detection
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高效液相色谱荧光检测法同时测定红曲米中两种红曲霉代谢物
DOI：
10.1016/j.foodchem.2011.01.004
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发表时间：
2011-08
期刊：
影响因子：
8.8
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Huang Z B;Xu Y;Zhang H;et al.
采用荧光检测反相高效液相色谱法测定红曲米中两种红曲霉代谢物红曲霉 A (MFA) 和红曲霉 B (MFB)。研究了提取和色谱分离的最佳条件。该方法通过以下性能标准进行了验证：线性、稳定性、检测限 (LOD)、定量 (LOQ) 等。该测定已成功用于测定从不同红曲菌种接种的 20 个样品中的 MFA 和 MFB。结果表明，这些样品中 MFA 和 MFB 的含量存在显着差异。样品 13 中 MFA 和 MFB 的含量较高，分别为 81.400 和 26.300mgg−1。样品 14 中的 MFA 和 MFB 含量也较低，分别为 0.010 和 0.003mgg−1。
A reversed-phase HPLC method with fluorescence detection for the determination of two Monascus metabolites, monasfluore A (MFA) and monasfluore B (MFB), in red yeast rice was carried out. Optimum conditions for the extraction and chromatographic separation were investigated. The method was validated through the following performance criteria: linearity, stability, limit of detection (LOD), quantification (LOQ), etc. This assay was successfully used for determination of the MFA and MFB in 20 samples inoculated from different Monascus sp. The results revealed that significant variations were demonstrated in the contents of the MFA and MFB in these samples. The high contents of both MFA and MFB in sample 13 were found to be 81.400 and 26.300mgg−1, respectively. The low contents of both MFA and MFB in sample 14 were also found to be 0.010 and 0.003mgg−1, respectively.
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影响因子：
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通讯作者：
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