EGR4 displays both a cell- and intracellular-specific localization pattern in the developing murine testis.
EGR4 displays both a cell- and intracellular-specific localization pattern in the developing murine testis.
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DOI：
10.1002/dvdy.22442
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发表时间：
2010-11
影响因子：
2.5
通讯作者：
Griswold, Michael
中科院分区：
文献类型：
--
作者：
Hogarth, Cathryn A.;Mitchell, Debra;Small, Christopher;Griswold, Michael
精子发生是精原干细胞向精子转化的一个复杂的细胞分化过程。转录因子EGR4的消除产生不育雄性小鼠，但哺乳动物睾丸中EGR4的表达和功能尚未得到充分研究。我们进行了原位杂交和免疫荧光，以确定Egr 4转录和蛋白定位在发育中的小鼠睾丸。EGR4在新生儿睾丸的生殖细胞和体细胞中均被检测到，但从青少年发育开始，EGR4对生精上皮内的生殖细胞具有特异性。EGR4在睾丸的特定细胞群中也显示出不同的细胞内定位模式。此外，Egr 4缺陷的睾丸小管从相对正常退化到支持细胞和未分化的精原细胞仅随着时间的推移。两者合计，这些数据表明，Egr 4可以调节精子发生在多个步骤，在分裂支持细胞，管周肌样细胞，减数分裂和延长单倍体生殖细胞群体的作用。
Spermatogenesis is an intricately regulated process of cellular differentiation transforming spermatogonial stem cells to spermatozoa. Elimination of the transcription factor EGR4 generates subfertile male mice yet the expression and function of EGR4 in the mammalian testis has yet to be fully investigated. We performed in situ hybridization and immunofluorescence to identify Egr4 transcript and protein localization in the developing murine testis. EGR4 was detected in both germ and somatic cells in the neonatal testis but was specific to germ cells inside the seminiferous epithelium from juvenile development onward. EGR4 also displayed distinct intracellular localization patterns within specific cell populations of the testis. In addition, Egr4-deficient testis tubules regress from relatively normal to Sertoli cell and undifferentiated spermatogonia only over time. Taken together, these data suggest that Egr4 may regulate spermatogenesis at multiple steps, with roles in the dividing Sertoli cells, peritubular myoid cells, and the meiotic and elongating haploid germ cell populations.
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