A complex choreography of cell movements shapes the vertebrate eye.
A complex choreography of cell movements shapes the vertebrate eye.
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DOI：
10.1242/dev.071407
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Chien CB
中科院分区：
文献类型：
--
作者：
Kwan KM;Otsuna H;Kidokoro H;Carney KR;Saijoh Y;Chien CB
视杯形态发生（OCM）产生了脊椎动物眼睛的基本结构。虽然它通常被描述为一系列的上皮折叠事件，这并不代表一个经验支持的模型。在这里，我们结合联合收割机四维成像与定制的细胞跟踪软件和光活化荧光标记，以确定斑马鱼OCM的细胞动力学基础。虽然细胞分裂有助于生长，但我们发现它不利于眼睛的形成。相反，OCM依赖于在预期的神经视网膜、视网膜色素上皮（RPE）和透镜之间协调的一组复杂的细胞运动。视泡外翻持续的时间比预期的要长;视泡伸长时细胞呈风车状移动，视网膜前体在内陷视杯边缘周围内卷。我们确定了意外的运动，特别是中央和周边视网膜，RPE和透镜。从细胞跟踪数据，我们产生视网膜，RPE和透镜子域的命运地图，揭示了新的邻接，可能会决定相应的发展信号事件。最后，我们发现，类似的运动也发生在鸡眼形态发生，这表明潜在的舞蹈是保守的脊椎动物。
Optic cup morphogenesis (OCM) generates the basic structure of the vertebrate eye. Although it is commonly depicted as a series of epithelial sheet folding events, this does not represent an empirically supported model. Here, we combine four-dimensional imaging with custom cell tracking software and photoactivatable fluorophore labeling to determine the cellular dynamics underlying OCM in zebrafish. Although cell division contributes to growth, we find it dispensable for eye formation. OCM depends instead on a complex set of cell movements coordinated between the prospective neural retina, retinal pigmented epithelium (RPE) and lens. Optic vesicle evagination persists for longer than expected; cells move in a pinwheel pattern during optic vesicle elongation and retinal precursors involute around the rim of the invaginating optic cup. We identify unanticipated movements, particularly of central and peripheral retina, RPE and lens. From cell tracking data, we generate retina, RPE and lens subdomain fate maps, which reveal novel adjacencies that might determine corresponding developmental signaling events. Finally, we find that similar movements also occur during chick eye morphogenesis, suggesting that the underlying choreography is conserved among vertebrates.
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影响因子：
2.7
作者：
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