Rapid mechanosensitive migration and dispersal of newly divided mesenchymal cells aid their recruitment into dermal condensates.
Rapid mechanosensitive migration and dispersal of newly divided mesenchymal cells aid their recruitment into dermal condensates.
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DOI：
10.1371/journal.pbio.3002316
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发表时间：
2023-09
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
胚胎间充质细胞分散在细胞外基质中，但可以合并形成具有关键发育作用的浓缩物。凝聚物内的细胞经历命运和形态变化，并诱导附近上皮细胞的细胞命运变化，以产生包括毛囊、羽毛或肠绒毛的结构。在这里，通过对小鼠和鸡胚胎皮肤进行成像，我们发现间充质细胞在早期间期经历了大部分的分散，在3小时的快速和持续的迁移，随后是长时间的低运动性驱动的刻板的位移过程中。新生间充质细胞的细胞分裂平面和升高的迁移速度和持久性是机械敏感的，与组织张力一致，并且依赖于活性WNT分泌。这种行为分散间充质细胞，并允许最近分裂的女儿长途跋涉进入真皮冷凝物，表明细胞周期亚阶段对核心间充质行为的意想不到的影响。胚胎间充质细胞分散在细胞外基质中，但可以合并形成具有关键发育作用的浓缩物。这项研究使用胚胎皮肤的实时成像来揭示间充质细胞在分裂后三小时内以快速和持久的方式迁移，有丝分裂的角度以及迁移的方向和速度由组织张力控制。
Embryonic mesenchymal cells are dispersed within an extracellular matrix but can coalesce to form condensates with key developmental roles. Cells within condensates undergo fate and morphological changes and induce cell fate changes in nearby epithelia to produce structures including hair follicles, feathers, or intestinal villi. Here, by imaging mouse and chicken embryonic skin, we find that mesenchymal cells undergo much of their dispersal in early interphase, in a stereotyped process of displacement driven by 3 hours of rapid and persistent migration followed by a long period of low motility. The cell division plane and the elevated migration speed and persistence of newly born mesenchymal cells are mechanosensitive, aligning with tissue tension, and are reliant on active WNT secretion. This behaviour disperses mesenchymal cells and allows daughters of recent divisions to travel long distances to enter dermal condensates, demonstrating an unanticipated effect of cell cycle subphase on core mesenchymal behaviour. Embryonic mesenchymal cells are dispersed within an extracellular matrix but can coalesce to form condensates with key developmental roles. This study uses live-imaging of embryonic skin to reveal that mesenchymal cells migrate in a rapid and persistent manner for three hours after division, with the angle of mitosis and direction and speed of migration controlled by tissue tension.
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影响因子：
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作者：
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