Neurotrophic peptides, ADNF-9 and NAP, prevent alcohol-induced apoptosis at midgestation in fetal brains of C57BL/6 mouse.
Neurotrophic peptides, ADNF-9 and NAP, prevent alcohol-induced apoptosis at midgestation in fetal brains of C57BL/6 mouse.
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DOI：
10.1007/s12031-012-9921-3
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发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Nkrumah-Abrokwah M
中科院分区：
文献类型：
--
作者：
Sari Y;Weedman JM;Nkrumah-Abrokwah M
已知产前酒精暴露会在不同的胚胎阶段诱导胎儿脑生长缺陷。本研究的重点是研究妊娠中期使用活动依赖性神经营养因子（ADNF）-9（一种来自活动依赖性神经营养因子的肽（SALLRSIPA））和NAP（一种来自活动依赖性神经保护蛋白的肽（NAPVSIPQ））对酒精诱导的细胞凋亡的神经保护作用。我们使用了一个已建立的胎儿酒精暴露小鼠模型。在胚胎第7天（E7），将体重匹配的妊娠雌性动物分配至以下组：（1）酒精流质饮食（ALC）组，（4.49%，v/v）乙醇来源的热量，（2）配对喂养（PF）对照组，（3）ALC联合腹腔注射（1.5 mg/kg）ADNF-9（4）ALC联合腹腔注射（5）PF流食+腹腔注射ADNF-9 PF/ADNF-9组（PF/ADNF-9组）;（6）PF/NAP组（PF/NAP组）。在第15天（E15），收集胎脑，称重，并测定TdT介导的dUTP缺口末端标记（TUNEL）染色。从E7至E15每天给予ADNF-9或NAP，同时暴露于PF或ALC液体饮食。我们的研究结果表明，NAP和ADNF-9显着防止酒精诱导的胎儿脑重量减轻。TUNEL染色法测定细胞凋亡; NAP或ADNF-9给药与酒精暴露一起显著防止酒精诱导的扣带回皮质和神经节隆起原基中TUNEL阳性细胞的增加。这些发现可能为预防妊娠期酒精中毒的潜在疗法铺平道路。
Prenatal alcohol exposure is known to induce fetal brain growth deficits at different embryonic stages. We focused this study on investigating the neuroprotective effects against alcohol-induced apoptosis at midgestation using activity-dependent neurotrophic factor (ADNF)-9, a peptide (SALLRSIPA) derived from activity-dependent neurotrophic factor, and NAP, a peptide (NAPVSIPQ) derived from activity-dependent neuroprotective protein. We used an established fetal alcohol exposure mouse model. On embryonic day 7 (E7), weight-matched pregnant females were assigned to the following groups: (1) ethanol liquid diet (ALC) group with 25 % (4.49 %, v/v) ethanol-derived calories, (2) pair-fed (PF) control group, (3) ALC combined with i.p. injections (1.5 mg/kg) of ADNF-9 (ALC/ADNF-9) group, (4) ALC combined with i.p. injections (1.5 mg/kg) of NAP (ALC/NAP) group, (5) PF liquid diet combined with i.p. injections of ADNF-9 (PF/ADNF-9) group, and (6) PF liquid diet combined with i.p. injections of NAP (PF/NAP) group. On day 15 (E15), fetal brains were collected, weighed, and assayed for TdT-mediated dUTP nick end labeling (TUNEL) staining. ADNF-9 or NAP was administered daily from E7 to E15 alongside PF or ALC liquid diet exposure. Our results show that NAP and ADNF-9 significantly prevented alcohol-induced weight reduction of fetal brains. Apoptosis was determined by TUNEL staining; NAP or ADNF-9 administration alongside alcohol exposure significantly prevented alcohol-induced increase in TUNEL-positive cells in primordium of the cingulate cortex and ganglionic eminence. These findings may pave the path toward potential therapeutics against alcohol intoxication during pregnancy stages.
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影响因子：
2.9
作者：
BARRON, S;GAGNON, WA;RILEY, EP
通讯作者：
RILEY, EP
DOI：
10.1111/j.1530-0277.1995.tb01624.x
发表时间：
1995-10-01
影响因子：
3.2
作者：
MIDDAUGH, LD;BOGGAN, WO
通讯作者：
BOGGAN, WO
影响因子：
2.3
作者：
BONTHIUS, DJ;GOODLETT, CR;WEST, JR
通讯作者：
WEST, JR
影响因子：
4.7
作者：
Bassan, M;Zamostiano, R;Gozes, I
通讯作者：
Gozes, I
影响因子：
2.9
作者：
KORNGUTH, SE;RUTLEDGE, JJ;YOUNG, B
通讯作者：
YOUNG, B