The size and culturability of patient-generated SARS-CoV-2 aerosol.
The size and culturability of patient-generated SARS-CoV-2 aerosol.
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DOI：
10.1038/s41370-021-00376-8
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发表时间：
2022-09
影响因子：
4.5
通讯作者：
Lowe JJ
中科院分区：
文献类型：
--
作者：
Santarpia JL;Herrera VL;Rivera DN;Ratnesar-Shumate S;Reid SP;Ackerman DN;Denton PW;Martens JWS;Fang Y;Conoan N;Callahan MV;Lawler JV;Brett-Major DM;Lowe JJ
COVID-19的气溶胶传播是正在进行的政策辩论的主题。表征COVID-19患者产生的气溶胶对于了解气溶胶在传播中的作用至关重要。我们调查了2020年4月入住混合急症病房的6名COVID-19患者的尺寸分级气溶胶中的病毒存在情况。从COVID-19阳性患者中收集了粒度分级的气溶胶样本和气溶胶粒度分布。对气溶胶样品进行病毒RNA分析，阳性样品在Vero E6细胞中培养。细胞的系列RT-PCR表明可能发生病毒复制的样品。还通过蛋白质印迹和透射电子显微镜（TEM）研究了病毒的存在。用rRT-PCR检测所有标本中SARS-CoV-2 RNA。三个样本确信地表明存在病毒复制，所有这些样本都来自收集的亚微米气溶胶。蛋白质印迹法表明在所有这些样品中存在病毒蛋白质，但其中一个样品中，并通过TEM观察到完整的病毒粒子。在亚微米气溶胶样本培养物中观察到的病毒复制提供了额外的证据，证明COVID-19可能通过空气传播。这些结果支持在医疗保健和公众中使用有效的呼吸保护来限制传播。
Aerosol transmission of COVID-19 is the subject of ongoing policy debate. Characterizing aerosol produced by people with COVID-19 is critical to understanding the role of aerosols in transmission. We investigated the presence of virus in size-fractioned aerosols from six COVID-19 patients admitted into mixed acuity wards in April of 2020. Size-fractionated aerosol samples and aerosol size distributions were collected from COVID-19 positive patients. Aerosol samples were analyzed for viral RNA, positive samples were cultured in Vero E6 cells. Serial RT-PCR of cells indicated samples where viral replication was likely occurring. Viral presence was also investigated by western blot and transmission electron microscopy (TEM). SARS-CoV-2 RNA was detected by rRT-PCR in all samples. Three samples confidently indicated the presence of viral replication, all of which were from collected sub-micron aerosol. Western blot indicated the presence of viral proteins in all but one of these samples, and intact virions were observed by TEM in one sample. Observations of viral replication in the culture of submicron aerosol samples provides additional evidence that airborne transmission of COVID-19 is possible. These results support the use of efficient respiratory protection in both healthcare and by the public to limit transmission.
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DOI：
10.1080/02786826.2021.1910137
发表时间：
2021-04-19
期刊：
Aerosol science and technology : the journal of the American Association for Aerosol Research
影响因子：
--
作者：
通讯作者：
--
影响因子：
8.4
作者：
Lednickya, John A.;Lauzardo, Michael;Wu, Chang-Yu
通讯作者：
Wu, Chang-Yu
影响因子：
6.7
作者：
Milton, Donald K.;Fabian, M. Patricia;McDevitt, James J.
通讯作者：
McDevitt, James J.
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
DOI：
10.1007/s10096-020-03913-9
发表时间：
2020-04-27
影响因子：
4.5
作者：
La Scola, Bernard;Le Bideau, Marion;Raoult, Didier
通讯作者：
Raoult, Didier