Comparison of the performance of aerosol sampling devices for measuring infectious SARS-CoV-2 aerosols.
Comparison of the performance of aerosol sampling devices for measuring infectious SARS-CoV-2 aerosols.
复制标题
DOI：
10.1080/02786826.2021.1910137
复制
发表时间：
2021-04-19
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
为了评估 SARS-CoV-2 气溶胶传播的风险，需要测量感染个体附近的空气传播病毒浓度、病毒在气溶胶中的持久性以及吸入后引起感染所需的病毒剂量。对于旨在量化这些参数的研究，需要使用气溶胶采样装置。最近的一些研究报告称，在临床环境中收集的空气样本中检测到了遗传物质和传染性 SARS-CoV-2 病毒。先前的研究表明，不同采样器收集和保存微生物感染性的效率可能会随着特定微生物的不同而变化。在本研究中，比较了八种常见的低流量气溶胶采样装置收集和保留小颗粒气溶胶中所含空气传播 SARS-CoV-2 传染性的能力。还评估了采样持续时间对感染性病毒回收率的影响。大多数测试的采样器在气溶胶中测得的传染性 SARS-CoV-2 浓度相似，但小型撞击器除外，该采样器测得的 SARS-CoV-2 浓度明显较低。此外，在测试的四个撞击器中的三个中，收集传染性病毒后通过设备的额外清洁气流导致病毒的传染性浓度随着时间的推移而降低，这表明病毒正在被灭活，并且这些设备可能不适合长时间采样。此外，随着时间的推移，样本中的 RNA 拷贝与在撞击器样本中观察到的传染性 SARS-CoV-2 的损失并不相符。这些数据可用于解释当前关于空气样本中 SARS-CoV-2 病毒载量的研究，并为未来研究中采样设备的选择提供信息。
To assess the risk of aerosol transmission of SARS-CoV-2, measurements of the airborne viral concentrations in proximity to infected individuals, the persistence of the virus in aerosols, and the dose of the virus needed to cause infection following inhalation are required. For studies aimed at quantifying these parameters, an aerosol sampling device needs to be employed. A number of recent studies have reported the detection of both genetic material and infectious SARS-CoV-2 virus in air samples collected in clinical settings. Previous studies have demonstrated that the efficiency of different samplers for collection and preservation of the infectivity of microorganisms can vary as a function of the specific microorganism. In the present study, the performance of eight common low-flow aerosol sampling devices were compared for their ability to collect and preserve the infectivity of airborne SARS-CoV-2 contained in small particle aerosols. The influence of sampling duration on recovery of infectious virus was also evaluated. Similar concentrations of infectious SARS-CoV-2 were measured in aerosols for the majority of the samplers tested, with the exception of the midget impingers, which measured significantly lower concentrations of SARS-CoV-2. Additionally, in three of the four impingers tested, additional clean airflow through the device following collection of infectious virus resulted in a decrease of the infectious concentration of virus over time, suggesting that virus was being inactivated and these devices may not be suitable for sampling for long durations. Further, RNA copies in the samples over time did not correspond with the losses of infectious SARS-CoV-2 observed in the impingers samples. These data can be utilized to inform interpretation of current studies on the SARS-CoV-2 viral loads in air samples, as well as inform sampling device selection in future studies.
登录
查看更多内容
DOI：
10.1016/j.jhin.2016.03.017
发表时间：
2016-07
期刊：
The Journal of hospital infection
影响因子：
--
作者：
Haig CW;Mackay WG;Walker JT;Williams C
通讯作者：
Williams C
影响因子：
8.4
作者：
Lednickya, John A.;Lauzardo, Michael;Wu, Chang-Yu
通讯作者：
Wu, Chang-Yu
影响因子：
6.7
作者：
Milton, Donald K.;Fabian, M. Patricia;McDevitt, James J.
通讯作者：
McDevitt, James J.
影响因子：
4.4
作者：
Mbareche, Hamza;Veiltette, Marc;Duchaine, Caroline
通讯作者：
Duchaine, Caroline
影响因子：
5.8
作者：
Fabian P;McDevitt JJ;Houseman EA;Milton DK
通讯作者：
Milton DK