Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy.
Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy.
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DOI：
10.1016/j.jconrel.2016.07.026
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发表时间：
2016-12-28
期刊：
影响因子：
--
通讯作者：
Al-Jamal KT
中科院分区：
文献类型：
--
作者：
Bai J;Wang JT;Mei KC;Al-Jamal WT;Al-Jamal KT
磁性药物靶向已被提出作为将治疗剂集中在靶点的手段，这种方法的成功已在许多研究中得到证明。然而，磁性载体在血管和肿瘤微循环中的行为仍不清楚。在这项工作中，我们利用聚合物磁性纳米胶囊(m-NCS)在肿瘤中进行磁靶向，并使用纤维共聚焦荧光显微镜(FCFM)在磁场暴露前、期间和之后动态显示它们在血管和肿瘤组织中的情况。我们的结果表明，m-NCs在肿瘤血管内的分布发生了巨大的变化，但在施加和去除磁场后，这种变化是可逆的。当肿瘤暴露在磁场中但没有破裂血管时，m-NC集中在血管壁附近，并以簇的形式停留。获得的FCFM图像提供了具有高空间分辨率但微创外科手术的m-NCS的活体微血管观察。这项在小鼠身上进行的概念验证描述性研究设想在微观分辨率下以非侵入性的方式跟踪和量化体内的纳米颗粒。
Magnetic drug targeting has been proposed as means of concentrating therapeutic agents at a target site and the success of this approach has been demonstrated in a number of studies. However, the behavior of magnetic carriers in blood vessels and tumor microcirculation still remains unclear. In this work, we utilized polymeric magnetic nanocapsules (m-NCs) for magnetic targeting in tumors and dynamically visualized them within blood vessels and tumor tissues before, during and after magnetic field exposure using fibered confocal fluorescence microscopy (FCFM). Our results suggested that the distribution of m-NCs within tumor vasculature changed dramatically, but in a reversible way, upon application and removal of a magnetic field. The m-NCs were concentrated and stayed as clusters near a blood vessel wall when tumors were exposed to a magnetic field but without rupturing the blood vessel. The obtained FCFM images provided in vivo in situ microvascular observations of m-NCs upon magnetic targeting with high spatial resolution but minimally invasive surgical procedures. This proof-of-concept descriptive study in mice is envisaged to track and quantify nanoparticles in vivo in a non-invasive manner at microscopic resolution.
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影响因子：
3.1
作者：
Derieppe, Marc;Yudina, Anna;Moonen, Chrit
通讯作者：
Moonen, Chrit
影响因子：
2.8
作者：
Faye, N.;Fournier, L.;Clement, O.
通讯作者：
Clement, O.
影响因子：
2.7
作者：
Jurgons, R.;Seliger, C.;Alexiou, C.
通讯作者：
Alexiou, C.
DOI：
10.1016/j.jconrel.2011.06.033
发表时间：
2011-11-07
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Chertok B;David AE;Yang VC
通讯作者：
Yang VC
影响因子：
14
作者：
Chertok, Beata;Moffat, Bradford A.;Yang, Victor C.
通讯作者：
Yang, Victor C.