Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii.
Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii.
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DOI：
10.3389/fmicb.2022.945108
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
变形链球菌（S. mutans）是导致龋齿的主要病原体之一。哥氏链球菌是牙菌斑的早期殖民者之一，可以与变形链球菌竞争生长。在目前的分析中，我们利用80株具有不同抗gordonii能力的变形链球菌临床分离株，探索了变形链球菌中抗gordonii的关键靶基因。主坐标分析显示，拮抗组和非拮抗组的遗传多样性差异显著。基因组比较显示，分别有33个和61个基因与变形链球菌抗gordonii呈正相关和负相关，rna测序（RNA-seq）结果显示，在拮抗组中，分别有11个和43个基因表达上调和下调。通过结合这些结果和抗smash分析，我们选择了16个基因进行qRT-PCR验证，其中SMU_137（苹果酸脱氢酶，mleS）、SMU_138（苹果酸渗透酶，mleP）、SMU_139（草酸脱羧酶，oxdC）和谷胱甘肽还原酶（SMU_140）的表达水平与RNA-seq结果一致。SMU_1315c-1317c （SMU_1315c转运相关基因）和SMU_1908c-1909c在拮抗组中分别下调和上调。相邻基因的表达模式密切相关，相关系数大于0.9。这些数据揭示了新的靶点（SMU_137-140， SMU_1315c-1317c和SMU_1908c-1909c），用于研究变形链球菌临床分离株中抗gordonii的关键基因簇。
Streptococcus mutans (S. mutans) is one of the primary pathogens responsible for dental caries. Streptococcus gordonii (S. gordonii) is one of the early colonizers of dental plaque and can compete with S. mutans for growth. In the present analysis, we explored key target genes against S. gordonii in S. mutans using 80 S. mutans clinical isolates with varying capabilities against S. gordonii. A principal coordinate analysis revealed significant genetic diversity differences between antagonistic and non-antagonistic groups. Genomic comparisons revealed 33 and 61 genes that were, respectively, positively and negatively correlated with S. mutans against S. gordonii, with RNA-sequencing (RNA-seq) highlighting 11 and 43 genes that were, respectively, upregulated and downregulated in the antagonistic group. Through a combination of these results and antiSMASH analysis, we selected 16 genes for qRT-PCR validation in which the expression levels of SMU_137 (malate dehydrogenase, mleS), SMU_138 (malate permease, mleP), SMU_139 (oxalate decarboxylase, oxdC), and SMU_140 (glutathione reductase) were consistent with RNA-seq results. SMU_1315c-1317c (SMU_1315c transport-related gene) and SMU_1908c-1909c were, respectively, downregulated and upregulated in the antagonistic group. The expression patterns of adjacent genes were closely related, with correlation coefficient values greater than 0.9. These data reveal new targets (SMU_137–140, SMU_1315c-1317c, and SMU_1908c-1909c) for investigating the critical gene clusters against S. gordonii in S. mutans clinical isolates.
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