Differential regulation of parvalbumin and calretinin interneurons in the prefrontal cortex during adolescence.
Differential regulation of parvalbumin and calretinin interneurons in the prefrontal cortex during adolescence.
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DOI：
10.1007/s00429-013-0508-8
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发表时间：
2014-01
影响因子：
3.1
通讯作者：
Tseng, Kuei Y.
中科院分区：
文献类型：
--
作者：
Caballero, Adriana;Flores-Barrera, Eden;Cass, Daryn K.;Tseng, Kuei Y.
在青春期过渡期，确定前额叶皮层（PFC）中单个GABA能成分的正常发育轨迹至关重要，因为局部GABA能中间神经元被认为在此发育窗口中发生的认知控制功能成熟中起重要作用。基于钙结合蛋白的表达，在PFC中已鉴定出3种不同的中间神经元亚型：小白蛋白（PV）阳性细胞、钙视网膜蛋白（CR）阳性细胞和钙结合蛋白（CB）阳性细胞。使用生物化学和组织化学的措施，我们发现，PV的蛋白质水平是最低的青少年（出生后一天-PD- 25-35），并在青春期（PD 45 -55）增加到类似于成年期（PD 65 -75）观察到的水平。相比之下，CR的蛋白质表达在成年动物中比幼年和青春期动物减少，而CB水平在这里研究的发育窗口中基本保持不变。半定量免疫染色分析显示，青少年期PV的上调和CR信号的丢失似乎是由于PV和CR阳性神经支配的变化，这是分离的PV和CR阳性细胞数量的轨迹。在突触水平上，我们的电生理数据显示，在青春期前后过渡期，PV阳性/快速发放的中间神经元上的自发性突触能突触输入的发育促进与前额叶PV信号的增加平行。相反，在PV阴性/非快速发放的中间神经元中，没有观察到年龄依赖性的突触传递变化。总之，这些研究结果强调，GABA能抑制性中间神经元在PFC在青春期进行动态的，细胞类型特异性重塑，并提供了一个发展的框架，了解GABA能电路的改变，发生在精神疾病。
Determining the normal developmental trajectory of individual GABAergic components in the prefrontal cortex (PFC) during the adolescent transition period is critical because local GABAergic interneurons are thought to play an important role in the functional maturation of cognitive control that occurs in this developmental window. Based on the expression of calcium-binding proteins, 3 distinctive subtypes of interneurons have been identified in the PFC: parvalbumin (PV)-, calretinin (CR)-, and calbindin (CB)-positive cells. Using biochemical and histochemical measures, we found that the protein level of PV is lowest in juveniles (postnatal day -PD- 25–35) and increases during adolescence (PD45–55) to levels similar to those observed in adulthood (PD65–75). In contrast, the protein expression of CR is reduced in adults compared to juvenile and adolescent animals, whereas CB levels remain mostly unchanged across the developmental window studied here. Semi-quantitative immunostaining analyses revealed that the periadolescent upregulation of PV and the loss of the CR signal appear to be attributable to changes in PV- and CR-positive innervation, which are dissociable from the trajectory of PV- and CR-positive cell number. At the synaptic level, our electrophysiological data revealed that a developmental facilitation of spontaneous glutamatergic synaptic inputs onto PV-positive/fast-spiking interneurons parallels the increase in prefrontal PV signal during the periadolescent transition. In contrast, no age-dependent changes in glutamatergic transmission were observed in PV-negative/non fast-spiking interneurons. Together, these findings emphasize that GABAergic inhibitory interneurons in the PFC undergo a dynamic, cell-type specific remodeling during adolescence and provide a developmental framework for understanding alterations in GABAergic circuits that occur in psychiatric disorders.
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影响因子：
4.7
作者：
Heng, Li-Jun;Markham, Julie A.;Tseng, Kuei Y.
通讯作者：
Tseng, Kuei Y.
影响因子：
34.7
作者：
Ascoli, Giorgio A.;Alonso-Nanclares, Lidia;Anderson, Stewart A.;Barrionuevo, German;Benavides-Piccione, Ruth;Burkhalter, Andreas;Buzsaki, Gyoergy;Cauli, Bruno;DeFelipe, Javier;Fairen, Alfonso;Feldmeyer, Dirk;Fishell, Gord;Fregnac, Yves;Freund, Tamas F.;Gardner, Daniel;Gardner, Esther P.;Goldberg, Jesse H.;Helmstaedter, Moritz;Hestrin, Shaul;Karube, Fuyuki;Kisvarday, Zoltan F.;Lambolez, Bertrand;Lewis, David A.;Marin, Oscar;Markram, Henry;Munoz, Alberto;Packer, Adam;Petersen, Carl C. H.;Rockland, Kathleen S.;Rossier, Jean;Rudy, Bernardo;Somogyi, Peter;Staiger, Jochen F.;Tamas, Gabor;Thomson, Alex M.;Toledo-Rodriguez, Maria;Wang, Yun;West, David C.;Yuste, Rafael
通讯作者：
Yuste, Rafael
影响因子：
4.6
作者：
Best JR;Miller PH
通讯作者：
Miller PH
影响因子：
8.2
作者：
Andersen, SL
通讯作者：
Andersen, SL
影响因子：
2.5
作者：
GIGUERE, M;GOLDMANRAKIC, PS
通讯作者：
GOLDMANRAKIC, PS