FOXL2 and DMRT1L Are Yin and Yang Genes for Determining Timing of Sex Differentiation in the Bivalve Mollusk Patinopecten yessoensis.
FOXL2 and DMRT1L Are Yin and Yang Genes for Determining Timing of Sex Differentiation in the Bivalve Mollusk Patinopecten yessoensis.
复制标题
FOXL2和DMRT1L是决定双壳类软体动物虾夷扇贝性别分化时间的阴阳基因
DOI：
10.3389/fphys.2018.01166
复制
发表时间：
2018
影响因子：
4
通讯作者：
Bao Z
中科院分区：
文献类型：
--
作者：
Li R;Zhang L;Li W;Zhang Y;Li Y;Zhang M;Zhao L;Hu X;Wang S;Bao Z
性别决定和分化一直是后生动物的研究热点。然而，人们对大多数软体动物何时以及如何发生性别分化知之甚少。在本研究中，我们对虾夷扇贝的性别分化进行了形态和分子相结合的研究。对5~13月龄幼鱼的性腺组织学观察表明，形态上的性别分化发生在10月龄。为了确定分子性别分化的开始，筛选了用于性别早期鉴定的分子标记。性别决定或分化的8个候选基因的性腺表达谱显示，只有2个基因表现出性别二态表达，其中FOXL2在卵巢中丰富，DMRT1L在睾丸中丰富。原位杂交结果显示，两者均在生殖细胞和卵泡细胞中检测到。因此，我们建立了用于扇贝性别鉴定的Log10(DMRT1L/FOXL2)，并证实了其在分化个体中的可行性。通过追踪其在5~13月龄幼体中的变化，确定了分子性分化时间：有些扇贝在9月上旬7月龄时分化，有些在12月下旬10月龄时分化。FOXL2和DMRT1L之间存在两种共表达模式：分化后预期的拮抗和分化前的意外协同。我们的结果表明，扇贝的性别分化与卵泡的形成同时发生，分子性别分化先于形态性别分化而建立。我们的研究将有助于更好地理解双壳类性别分化的分子机制。
Sex determination and differentiation have long been a research hotspot in metazoans. However, little is known about when and how sex differentiation occurs in most mollusks. In this study, we conducted a combined morphological and molecular study on sex differentiation in the Yesso scallop Patinopecten yessoensis. Histological examination on gonads from 5- to 13-month-old juveniles revealed that the morphological sex differentiation occurred at 10 months of age. To determine the onset of molecular sex differentiation, molecular markers were screened for early identification of sex. The gonadal expression profiles of eight candidate genes for sex determination or differentiation showed that only two genes displayed sexually dimorphic expression, with FOXL2 being abundant in ovaries and DMRT1L in testes. In situ hybridization revealed that both of them were detected in germ cells and follicle cells. We therefore developed LOG10(DMRT1L/FOXL2) for scallop sex identification and confirmed its feasibility in differentiated individuals. By tracing its changes in 5- to 13-month-old juveniles, molecular sex differentiation time was determined: some scallops differentiate early in September when they are 7 months old, and some do late in December when they are 10 months old. Two kinds of coexpression patterns were found between FOXL2 and DMRT1L: expected antagonism after differentiation and unexpected coordination before differentiation. Our results revealed that scallop sex differentiation co-occurs with the formation of follicles, and molecular sex differentiation is established prior to morphological sex differentiation. Our study will assist in a better understanding of the molecular mechanism underlying bivalve sex differentiation.
登录
查看更多内容
卵巢和睾丸的转录组测序和比较分析鉴定了扇贝扇贝性别相关的潜在关键基因和通路
影响因子：
3
作者：
Li, Yangping;Zhang, Lingling;Bao, Zhenmin
通讯作者：
Bao, Zhenmin
影响因子：
64.8
作者：
Matson, Clinton K.;Murphy, Mark W.;Sarver, Aaron L.;Griswold, Michael D.;Bardwell, Vivian J.;Zarkower, David
通讯作者：
Zarkower, David
DOI：
10.1016/j.cbpa.2008.09.019
发表时间：
2009-02-01
影响因子：
2.3
作者：
Naimi, Amine;Martinez, Anne-Sophie;Sourdaine, Pascal
通讯作者：
Sourdaine, Pascal
影响因子：
1.6
作者：
Li Hailong;Liu Jianguo;Zhang Zhifeng
通讯作者：
Zhang Zhifeng
RNA测序揭示了鸡在性腺分化之前的性二态基因表达，并允许对W-染色体的全面注释。
影响因子：
12.3
作者：
Ayers KL;Davidson NM;Demiyah D;Roeszler KN;Grützner F;Sinclair AH;Oshlack A;Smith CA
通讯作者：
Smith CA