The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species.
The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species.
复制标题
DOI：
10.1186/s13059-016-1049-2
复制
发表时间：
2016-09-22
期刊：
影响因子：
12.3
通讯作者：
Handler AM
中科院分区：
文献类型：
--
作者：
Papanicolaou A;Schetelig MF;Arensburger P;Atkinson PW;Benoit JB;Bourtzis K;Castañera P;Cavanaugh JP;Chao H;Childers C;Curril I;Dinh H;Doddapaneni H;Dolan A;Dugan S;Friedrich M;Gasperi G;Geib S;Georgakilas G;Gibbs RA;Giers SD;Gomulski LM;González-Guzmán M;Guillem-Amat A;Han Y;Hatzigeorgiou AG;Hernández-Crespo P;Hughes DS;Jones JW;Karagkouni D;Koskinioti P;Lee SL;Malacrida AR;Manni M;Mathiopoulos K;Meccariello A;Munoz-Torres M;Murali SC;Murphy TD;Muzny DM;Oberhofer G;Ortego F;Paraskevopoulou MD;Poelchau M;Qu J;Reczko M;Robertson HM;Rosendale AJ;Rosselot AE;Saccone G;Salvemini M;Savini G;Schreiner P;Scolari F;Siciliano P;Sim SB;Tsiamis G;Ureña E;Vlachos IS;Werren JH;Wimmer EA;Worley KC;Zacharopoulou A;Richards S;Handler AM
地中海实蝇（Medfly），Ceratitis capitata，是一种主要的破坏性昆虫害虫，由于其广泛的宿主范围，其中包括数百种水果和蔬菜。它表现出独特的能力，入侵和适应整个世界的热带和亚热带地区的生态位，虽然地中海果蝇的侵扰已被预防和控制的不育昆虫技术（SIT）的一部分，综合害虫管理计划（IPM）。地中海实蝇的遗传分析和操纵一直受到深入研究，以努力提高SIT效力和IPM控制的其他方面。对来自近交20代的品系的成蝇的479 Mb地中海实蝇基因组进行测序。实现了具有45.7kb的重叠群N50和4.06Mb的支架N50的高质量组装。对1800多种信使RNA的深入研究显示了与入侵和宿主适应相关的特定基因扩增，包括化学感受、毒素和杀虫剂代谢、角质层蛋白、视蛋白和水通道蛋白的基因家族。我们确定了IPM控制相关的基因，包括改善SIT所需的基因。地中海果蝇基因组序列提供了对最严重和最广泛的农业害虫之一的生物学的重要见解。这方面的知识应显着推进的手段，控制的大小和入侵潜力的地中海果蝇种群。它与果蝇以及其他对农业和人类健康很重要的昆虫物种的密切关系，将进一步对昆虫基因组进行比较功能和结构研究，从而拓宽我们对基因家族进化的理解。本文的在线版本（doi：10.1186/s13059-016-1049-2）包含补充材料，可供授权用户使用。
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (SIT) as part of integrated pest management programs (IPMs). The genetic analysis and manipulation of medfly has been subject to intensive study in an effort to improve SIT efficacy and other aspects of IPM control. The 479 Mb medfly genome is sequenced from adult flies from lines inbred for 20 generations. A high-quality assembly is achieved having a contig N50 of 45.7 kb and scaffold N50 of 4.06 Mb. In-depth curation of more than 1800 messenger RNAs shows specific gene expansions that can be related to invasiveness and host adaptation, including gene families for chemoreception, toxin and insecticide metabolism, cuticle proteins, opsins, and aquaporins. We identify genes relevant to IPM control, including those required to improve SIT. The medfly genome sequence provides critical insights into the biology of one of the most serious and widespread agricultural pests. This knowledge should significantly advance the means of controlling the size and invasive potential of medfly populations. Its close relationship to Drosophila, and other insect species important to agriculture and human health, will further comparative functional and structural studies of insect genomes that should broaden our understanding of gene family evolution. The online version of this article (doi:10.1186/s13059-016-1049-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
11.8
作者：
Boutros, M;Agaisse, H;Perrimon, N
通讯作者：
Perrimon, N
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
4.1
作者：
Arouri, Rabeh;Le Goff, Gaelle;Ortego, Felix
通讯作者：
Ortego, Felix
影响因子：
2.6
作者：
Bonizzoni, M.;Gomulski, L. M.;Gasperi, G.
通讯作者：
Gasperi, G.
影响因子：
7
作者：
Cantarel, Brandi L.;Korf, Ian;Yandell, Mark
通讯作者：
Yandell, Mark