Hyaluronic Acid Hydrogel Modified with Nogo-66 Receptor Antibody and Poly(L-Lysine) Enhancement of Adherence and Survival of Primary Hippocampal Neurons
Hyaluronic Acid Hydrogel Modified with Nogo-66 Receptor Antibody and Poly(L-Lysine) Enhancement of Adherence and Survival of Primary Hippocampal Neurons
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Nogo-66 受体抗体和聚赖氨酸修饰的透明质酸水凝胶增强原代海马神经元的粘附和存活
DOI：
10.1177/0883911509102266
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发表时间：
2009-05
影响因子：
1.7
通讯作者：
Wei, Yue-Teng
中科院分区：
文献类型：
--
作者：
Sun, Xiao-Dan;He, Yu;Cui, Fu-Zhai;Liu, Bing-Fang;Xia, Xue;Xu, Qun-Yuan;Wei, Yue-Teng
用聚赖氨酸（PLL）和Nogo-66受体抗体（抗NgR）修饰透明质酸（HA）水凝胶，以促进中枢神经系统（CNS）损伤的修复。用X射线光电子能谱（XPS）对PLL的固定化进行了表征，用免疫荧光法对抗NgR的固定化进行了研究。通过培养原代海马神经元，分析这种改性水凝胶的细胞相容性。不同的修饰影响神经元的数量和形态;与未修饰的水凝胶培养物相比，修饰的HA水凝胶培养的原代海马神经元表现出多极和双极形态。用PLL和抗NgR修饰的HA水凝胶培养获得的神经元数量几乎是用仅用PLL或抗NgR修饰的HA培养的神经元数量的两倍。这种现象是由于PLL和抗NgR对神经元的协同作用。这种新的水凝胶体系的特性，包括孔结构，吸水性，水解降解时，与PLL或antiNgR分别改性的水凝胶相比，没有太大的变化。预期这种改性的HA水凝胶具有作为CNS组织工程材料的潜力。
Hyaluronic acid (HA) hydrogel was modified with poly(L-lysine) (PLL) and Nogo-66 Receptor antibody (antiNgR) to enhance the repair of central nervous system (CNS) injuries. The immobilization of PLL was characterized by X-ray photoelectron spectroscopy (XPS) and the immobilization of antiNgR was studied by immunofluorescence. The cytocompatibility of this modified hydrogel was analyzed by culturing primary hippocampal neurons. The quantity and morphology of the neurons were influenced by different modifications; the primary hippocampal neurons cultured with modified HA hydrogel exhibited multipolar and bipolar morphology were compared with unmodified hydrogel cultures. The number of neurons obtained by culturing with HA hydrogel modified with both PLL and antiNgR was almost twice the number of neurons cultured with HA modified with only PLL or antiNgR. This phenomenon was attributed to the collaborative effect of PLL and antiNgR on the neurons. The characteristics of this new hydrogel system, including pore structure, water absorption, hydrolysis degradation did not change much when compared with the hydrogel modified with PLL or antiNgR, respectively. It is expected that this modified HA hydrogel has potential as a CNS tissue engineering material.
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影响因子：
4.2
作者：
Joosten, EAJ;Veldhuis, WB;Hamers, FPT
通讯作者：
Hamers, FPT
影响因子：
3.3
作者：
Hou, S;Tian, W;Zhao, C
通讯作者：
Zhao, C
影响因子：
2.3
作者：
M. Vert;Y. Doi;K. Hellwich;Michael Hess;Philip Hodge;P. Kubisa;M. Rinaudo;F. Schué
通讯作者：
M. Vert;Y. Doi;K. Hellwich;Michael Hess;Philip Hodge;P. Kubisa;M. Rinaudo;F. Schué
影响因子：
4.2
作者：
Shi, RY;Borgens, RB;Blight, AR
通讯作者：
Blight, AR
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP