Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets.
Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets.
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DOI：
10.3791/64413
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发表时间：
2022-09-13
影响因子：
1.2
通讯作者：
Adebiyi, Adebowale
中科院分区：
文献类型：
--
作者：
Fanous, Mina S.;Afolabi, Jeremiah M.;Michael, Olugbenga S.;Falayi, Olufunke O.;Iwhiwhu, Samson A.;Adebiyi, Adebowale
经皮测量肾小球滤过率（GFR）已被用于评估有意识动物的肾功能。该技术已在啮齿类动物中建立，用于研究急性肾损伤和慢性肾脏疾病。然而，使用透皮系统测量GFR尚未在猪身上得到验证，猪的肾脏系统与人类相似。因此，我们研究了脓毒症对麻醉和机械通气新生猪透皮GFR的影响。采用盲肠结扎穿刺法（CLP）诱导多微生物脓毒症。由微型荧光传感器组成的透皮GFR测量系统附着在猪剃毛的皮肤上，以测定静脉注射GFR示踪剂异硫氰酸荧光素（FITC）共轭辛曲霉素的清除率。我们的结果显示，clp后12小时，血清肌酐升高，GFR降低。这项研究首次证明了透皮GFR方法在机械通气新生猪肾功能测定中的应用。
Transdermal measurement of glomerular filtration rate (GFR) has been used to evaluate kidney function in conscious animals. This technique is well established in rodents to study acute kidney injury and chronic kidney disease. However, GFR measurement using the transdermal system has not been validated in pigs, a species with a similar renal system to humans. Hence, we investigated the effect of sepsis on transdermal GFR in anesthetized and mechanically ventilated neonatal pigs. Polymicrobial sepsis was induced by cecal ligation and puncture (CLP). The transdermal GFR measurement system consisting of a miniaturized fluorescence sensor was attached to the pig’s shaved skin to determine the clearance of fluorescein-isothiocyanate (FITC) conjugated sinistrin, an intravenously injected GFR tracer. Our results show that at 12 h post-CLP, serum creatinine increased with a decrease in GFR. This study demonstrates, for the first time, the utility of the transdermal GFR approach in determining renal function in mechanically ventilated, neonatal pigs.
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影响因子：
56.9
作者：
GUTMAN, Y;GOTTSCHALK, CW;LASSITER, WE
通讯作者：
LASSITER, WE
DOI：
10.1186/2047-1440-1-8
发表时间：
2012-08-16
期刊：
Transplantation research
影响因子：
--
作者：
Kobayashi E;Hishikawa S;Teratani T;Lefor AT
通讯作者：
Lefor AT
影响因子：
3
作者：
Gattineni, Jyothsna;Baum, Michel
通讯作者：
Baum, Michel
影响因子：
4.8
作者：
Ayuso, Miriam;Irwin, Rachelle;Van Ginneken, Chris
通讯作者：
Van Ginneken, Chris
影响因子：
1
作者：
Fusellier, Marion;Desfontis, Jean-Claude;Gogny, Marc
通讯作者：
Gogny, Marc