Diagnosis of Helicobacter spp. infection in canine stomach.
Diagnosis of Helicobacter spp. infection in canine stomach.
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DOI：
10.4142/jvs.2007.8.2.139
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发表时间：
2007-06
影响因子：
1.8
通讯作者：
Sailasuta A
中科院分区：
文献类型：
--
作者：
Prachasilpchai W;Nuanualsuwan S;Chatsuwan T;Techangamsuwan S;Wangnaitham S;Sailasuta A
对 2003 年 4 月至 2004 年 6 月提交给朱拉隆功大学兽医学院病理学系的尸检犬的贲门、眼底、体部和幽门窦活检样本共计 75 份进行了研究。本研究的目的是确定螺杆菌属的流行情况。通过聚合酶链反应 (PCR) 与组织化学与免疫组织化学 (IHC) 进行比较，并将这些诊断方法与受感染狗的临床意义联系起来。组织病理学结果显示，60.0%（45/75）的样本呈阳性，其中轻度胃炎占64.44%（29/45），中度胃炎占11.11%（5/45），重度胃炎占24.44%（11/45）。无组织病理学病变的比例为40.0%（30/75）。螺杆菌属18.67%（14/75）位于管腔隐窝，22.67%（17/75）位于胃凹，21.33%（16/75）位于胃腺，8%（6/75）位于胃上皮。螺杆菌属阳性样本的百分比。通过苏木精和伊红染色 (H&E)、Warthin Starry 染色 (WSS)、兔多克隆抗 H.IHC 进行诊断。幽门螺杆菌抗体和PCR分别为17.3%（13/75）、46.7%（35/75）、30.7%（23/75）和10.7%（8/75）。胃部分的组织病理学变化没有观察到显着差异（p>0.05）。螺杆菌属的诊断。 PCR 检测结果与 WSS 和 IHC 检测结果没有显着差异 (p>0.05)。使用 H&E、WSS 和 IHC 进行的病理研究之间没有关系，尤其是 PCR 与螺杆菌属临床症状之间没有关系。犬胃部感染（p>0.05）。本研究揭示了螺杆菌属的相关性水平存在显着差异。 H&E 和 WSS 之间的检测 (p<0.001)。结果表明，诊断螺杆菌的首选方法。犬胃部感染取决于研究目的和适当的标本采集。
A total of 75 biopsied samples of cardia, fundus, body, and pyloric antrum from necropsied dogs that were submitted to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University from April 2003 to June 2004 were investigated. The objectives of this study were to determine the prevalence of Helicobacter spp. in canine stomach by polymerase chain reaction (PCR) in comparison to histochemistry versus immunohistochemistry (IHC), and to correlate these diagnostic methods with the clinical significance in infected dogs. Histopathological results revealed 60.0% (45/75) of samples to be positive, and consisted of mild gastritis in 64.44% (29/45), moderate gastritis in 11.11% (5/45), and severe gastritis in 24.44% (11/45). The proportion showing no histopathological lesions was 40.0% (30/75). Helicobacter spp. were localized to the luminal crypt in 18.67% (14/75), gastric pit in 22.67% (17/75), gastric gland in 21.33% (16/75), and gastric epithelium in 8% (6/75). The percentages of positive samples of Helicobacter spp. diagnosed by hematoxylin and eosin stain (H&E), Warthin Starry stain (WSS), IHC with rabbit polyclonal anti-H. pylori antibody, and PCR were 17.3% (13/75), 46.7% (35/75), 30.7% (23/75), and 10.7% (8/75), respectively. No significant differences weree observed in histopathological changes in portions of the stomach (p>0.05). The diagnosis of Helicobacter spp. by PCR in comparison to that by WSS and IHC was not significantly different (p>0.05). There were no relationships between pathological studies using H&E, WSS, and IHC, and especially between PCR and clinical signs of Helicobacter spp. infections in canine stomachs (p>0.05). The present study revealed significantly different levels of correlation for Helicobacter spp. detection between H&E and WSS (p<0.001). Results indicate that the method of choice for diagnosis of Helicobacter spp. infection in canine stomach is dependent on the purpose of study and appropriate specimen collection.
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影响因子：
6
作者：
Esteves, MI;Schrenzel, MD;Fox, JG
通讯作者：
Fox, JG
影响因子：
9.4
作者：
Riley, LK;Franklin, CL;BeschWilliford, C
通讯作者：
BeschWilliford, C
影响因子：
3.4
作者：
AshtonKey, M;Diss, TC;Isaacson, PG
通讯作者：
Isaacson, PG
影响因子：
2.4
作者：
EATON, KA;RADIN, MJ;MORGAN, DR
通讯作者：
MORGAN, DR
影响因子：
9.4
作者：
LAGE, AP;GODFROID, E;GLUPCZYNSKI, Y
通讯作者：
GLUPCZYNSKI, Y