Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch.
Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch.
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DOI：
10.3390/nu10020144
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发表时间：
2018-01-29
期刊：
影响因子：
5.9
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Nishimura N;Tanabe H;Komori E;Sasaki Y;Inoue R;Yamamoto T
具有低氧化还原电位的氢分子（H2）是通过结肠发酵产生的。我们研究了喂食高直链玉米淀粉 (HAS) 的大鼠门静脉中 H2 浓度的增加是否有助于减轻氧化应激，以及移植高 H2 产生的大鼠结肠微生物群是否可以将低 H2 产生大鼠 (LG) 转变为高 H2 产生大鼠 (HG)。实验 1 和实验 2 中，大鼠分别喂食 20% HAS 饮食 10 天和 13 天。 10天后（实验1），大鼠接受肝脏缺血再灌注（IR）手术。然后将大鼠根据门静脉氢气浓度分为五分位数。随着门静脉 H2 浓度的增加，血浆丙氨酸转氨酶活性和肝氧化型谷胱甘肽浓度显着降低。实验2中，在第3天和第4天将来自HG（移植组）或生理盐水（对照组）的微生物群口服接种到LG中。第13天时，移植组的门静脉H2浓度显着高于对照组，并且与双歧杆菌属、异杆菌属和副杆菌属呈正相关，与拟杆菌属、瘤胃球菌属和埃希氏菌属呈负相关。总之，来自 HG 的微生物群的移植导致 LG 中稳定、高产氢气，由此产生的高产氢气有助于缓解氧化应激。
The hydrogen molecule (H2), which has low redox potential, is produced by colonic fermentation. We examined whether increased H2 concentration in the portal vein in rats fed high amylose maize starch (HAS) helped alleviate oxidative stress, and whether the transplantation of rat colonic microbiota with high H2 production can shift low H2-generating rats (LG) to high H2-generating rats (HG). Rats were fed a 20% HAS diet for 10 days and 13 days in experiments 1 and 2, respectively. After 10 days (experiment 1), rats underwent a hepatic ischemia–reperfusion (IR) operation. Rats were then categorized into quintiles of portal H2 concentration. Plasma alanine aminotransferase activity and hepatic oxidized glutathione concentration were significantly lower as portal H2 concentration increased. In experiment 2, microbiota derived from HG (the transplantation group) or saline (the control group) were orally inoculated into LG on days 3 and 4. On day 13, portal H2 concentration in the transplantation group was significantly higher compared with the control group, and positively correlated with genera Bifidobacterium, Allobaculum, and Parabacteroides, and negatively correlated with genera Bacteroides, Ruminococcus, and Escherichia. In conclusion, the transplantation of microbiota derived from HG leads to stable, high H2 production in LG, with the resultant high production of H2 contributing to the alleviation of oxidative stress.
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影响因子：
3.7
作者：
Di Luccia B;Crescenzo R;Mazzoli A;Cigliano L;Venditti P;Walser JC;Widmer A;Baccigalupi L;Ricca E;Iossa S
通讯作者：
Iossa S
影响因子：
56.9
作者：
Li, Simone S.;Zhu, Ana;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1016/j.bbrc.2008.05.165
发表时间：
2008-08-15
影响因子：
3.1
作者：
Hayashida, Kentaro;Sano, Motoaki;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
影响因子：
4.4
作者：
Falony, Gwen;Lazidou, Katerina;De Vuyst, Luc
通讯作者：
De Vuyst, Luc
影响因子：
2.4
作者：
Inoue R;Ohue-Kitano R;Tsukahara T;Tanaka M;Masuda S;Inoue T;Yamakage H;Kusakabe T;Hasegawa K;Shimatsu A;Satoh-Asahara N
通讯作者：
Satoh-Asahara N