3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs.
3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs.
复制标题
执行样品纯化和多重QRT-PCR进行早期癌症检测的3D微型版本，并确认特定RNA。
DOI：
10.1038/s41598-018-35772-y
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发表时间：
2018-11-30
影响因子：
4.6
通讯作者：
Ikuta K
中科院分区：
文献类型：
--
作者：
Kimura Y;Ikeuchi M;Inoue Y;Ikuta K
microRNA表达分析是癌症早期检测的重要筛查工具。在这项研究中，我们开发了两种便携式三维微设备，用于通过microRNA纯化和qRT-PCR进行多个单重RNA表达分析，作为床旁检测的原型。这些微器件由几种称为“化学IC芯片”的模块组成。我们成功地减少了加热面积和荧光观察面积，降低了反应所需的能量，并提高了设备中所有系统的便携性。该纯化微装置可使用FTA荧光卡系统从样品中纯化microRNA。安装在两个装置上的一次性反应器模块通过使用简单的程序使100 μ m厚的聚丙烯膜变形而容易地制造。qRT-PCR微型装置可以对小体积的样品进行反应。我们使用纯化微装置从HepG 2肝癌细胞系中纯化microRNA，并证实了miR-224的表达水平，miR-224是肝癌的潜在生物标志物。此外，当我们在qRT-PCR微型装置中进行qRT-PCR时，我们观察到荧光强度增加。因此，这两个开发的微型设备显示出作为早期癌症检测的新的便携式工具的前景。
MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA expression analysis by microRNA purification and qRT-PCR as a prototype for point-of-care testing. These microdevices are composed of several types of modules termed ‘chemical IC chips’. We successfully reduced the heating area and fluorescence observation area, reduced the energy required for the reaction, and improved the portability of all systems in the devices. The purification microdevice could purify the microRNA from the sample using the FTA elute card system. The disposable reactor module mounted on both devices was easily fabricated by deforming a 100-μm-thick polypropylene film using an uncomplicated procedure. The qRT-PCR microdevice could perform reactions for samples of small volume. We purified microRNA from the HepG2 liver cancer cell line using the purification microdevice and confirmed the expression level of miR-224, which is a potential biomarker for liver cancer. Furthermore, we observed an increase in the fluorescence intensity when we performed qRT-PCR in the qRT-PCR microdevice. Therefore, the two developed microdevices show promise as a new portable tool for early cancer detection.
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影响因子：
3.7
作者：
Ogata-Kawata H;Izumiya M;Kurioka D;Honma Y;Yamada Y;Furuta K;Gunji T;Ohta H;Okamoto H;Sonoda H;Watanabe M;Nakagama H;Yokota J;Kohno T;Tsuchiya N
通讯作者：
Tsuchiya N
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP
使用微流控机器人打印用于单细胞逆转录定量 PCR 测定的二维液滴阵列
影响因子：
4.6
作者：
Zhu Y;Zhang YX;Liu WW;Ma Y;Fang Q;Yao B
通讯作者：
Yao B
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
64.5
作者：
LEE, RC;FEINBAUM, RL;AMBROS, V
通讯作者：
AMBROS, V