Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein.
Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein.
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DOI：
10.1016/j.virusres.2013.05.004
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发表时间：
2013-09
期刊：
影响因子：
5
通讯作者：
Takada, Ayato
中科院分区：
文献类型：
--
作者：
Changula, Katendi;Yoshida, Reiko;Noyori, Osamu;Marzi, Andrea;Miyamoto, Hiroko;Ishijima, Mari;Yokoyama, Ayaka;Kajihara, Masahiro;Feldmann, Heinz;Mweene, Aaron S.;Takada, Ayato
丝状病毒（丝状病毒科中埃博拉病毒属和马尔堡病毒属的病毒）在人类和非人灵长类动物中引起严重的出血热。诊断和疫情控制需要快速、高灵敏度和可靠的丝状病毒特异性检测。病毒蛋白中抗原位点的表征可以帮助开发病毒抗原检测测定，例如基于免疫层析的快速诊断。我们产生了一组小鼠单克隆抗体（mAb）的埃博拉病毒的核蛋白（NP）属于物种扎伊尔埃博拉病毒。根据其特异性和与埃博拉病毒属其他物种的交叉反应性，将mAb分为7组。使用对应于埃博拉病毒NP序列的合成肽，将mAb结合位点定位到NP的C-末端一半中的七个抗原区域，包括目前已知的所有五种埃博拉病毒物种中的两个高度保守区域。此外，我们成功地产生了种特异性兔抗血清的合成肽预测代表独特的丝状病毒B细胞表位。我们的数据为埃博拉病毒抗原检测方法的发展提供了有用的信息。
Filoviruses (viruses in the genus Ebolavirus and Marburgvirus in the family Filoviridae) cause severe haemorrhagic fever in humans and nonhuman primates. Rapid, highly sensitive, and reliable filovirus-specific assays are required for diagnostics and outbreak control. Characterisation of antigenic sites in viral proteins can aid in the development of viral antigen detection assays such immunochromatography-based rapid diagnosis. We generated a panel of mouse monoclonal antibodies (mAbs) to the nucleoprotein (NP) of Ebola virus belonging to the species Zaire ebolavirus. The mAbs were divided into seven groups based on the profiles of their specificity and cross-reactivity to other species in the Ebolavirus genus. Using synthetic peptides corresponding to the Ebola virus NP sequence, the mAb binding sites were mapped to seven antigenic regions in the C-terminal half of the NP, including two highly conserved regions among all five Ebolavirus species currently known. Furthermore, we successfully produced species-specific rabbit antisera to synthetic peptides predicted to represent unique filovirus B-cell epitopes. Our data provide useful information for the development of Ebola virus antigen detection assays.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
10.1128/cdli.10.1.83-87.2003
发表时间：
2003-01-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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