A migrating ciliary gate compartmentalizes the site of axoneme assembly in Drosophila spermatids.
A migrating ciliary gate compartmentalizes the site of axoneme assembly in Drosophila spermatids.
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DOI：
10.1016/j.cub.2014.09.047
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发表时间：
2014-11-17
期刊：
影响因子：
9.2
通讯作者：
Avidor-Reiss, Tomer
中科院分区：
文献类型：
--
作者：
Basiri, Marcus L.;Ha, Andrew;Chadha, Abhishek;Clark, Nicole M.;Polyanovsky, Andrey;Cook, Boaz;Avidor-Reiss, Tomer
在大多数细胞中，纤毛形成于与细胞质分离的隔室中。进入纤毛室是由位于纤毛基部的一个称为过渡区的专门门调节的。移行区与纤毛基部的多个结构密切相关，包括中心粒、轴丝和纤毛膜。然而，这些结构对睫状门的贡献仍不清楚。在这里，我们报告说，在果蝇精子细胞，一个保守的模块的过渡区蛋白突变的Meckel-Gruber综合征（MKS），包括Cep 290，Mks 1，B 9d 1，和B 9d 2包括纤毛门，不断迁移远离中心粒区隔的轴丝尖端。我们发现，Cep 290是必不可少的过渡区组成，区室化的轴丝尖端，和轴丝的完整性，并发现MKS蛋白质也划定了一个中心粒独立的隔间在小鼠精子细胞。我们的研究结果表明，睫状门可以迁移远离纤毛的基础，从而独立于中心粒和静态相互作用的轴丝区室化的轴丝组装的网站。
In most cells, the cilium is formed within a compartment separated from the cytoplasm. Entry into the ciliary compartment is regulated by a specialized gate located at the base of the cilium in a region known as the transition zone. The transition zone is closely associated with multiple structures of the ciliary base including the centriole, axoneme, and ciliary membrane. However, the contribution of these structures to the ciliary gate remains unclear. Here, we report that in Drosophila spermatids, a conserved module of transition zone proteins mutated in Meckel-Gruber Syndrome (MKS) including Cep290, Mks1, B9d1, and B9d2 comprise a ciliary gate that continuously migrates away from the centriole to compartmentalize the growing axoneme tip. We show that Cep290 is essential for transition zone composition, compartmentalization of the axoneme tip, and axoneme integrity, and find that MKS proteins also delimit a centriole-independent compartment in mouse spermatids. Our findings demonstrate that the ciliary gate can migrate away from the base of the cilium, thereby functioning independently of the centriole and of a static interaction with the axoneme to compartmentalize the site of axoneme assembly.
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果蝇 chibby 是基体形成和纤毛发生所必需的，但不是 Wg 信号传导所必需的。
DOI：
10.1083/jcb.201109148
发表时间：
2012-04-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Enjolras C;Thomas J;Chhin B;Cortier E;Duteyrat JL;Soulavie F;Kernan MJ;Laurençon A;Durand B
通讯作者：
Durand B
影响因子：
10.5
作者：
Reiter, JF;Skarnes, WC
通讯作者：
Skarnes, WC
影响因子：
21.3
作者：
Chih, Ben;Liu, Peter;Peterson, Andrew S.
通讯作者：
Peterson, Andrew S.
影响因子：
2.3
作者：
Malicki J;Avidor-Reiss T
通讯作者：
Avidor-Reiss T
影响因子：
4
作者：
Ma L;Jarman AP
通讯作者：
Jarman AP